Han, Sang-Soo Lee, Dong-Eun Shim, Hye-Eun Lee, Sangmin Jung, Taekhee Oh, Jung-Hwa Lee, Hyang-Ae Moon, Sung-Hwan Jeon, Jongho Yoon, Seokjoo Kim, Kwangmeyung Kang, Sun-Woong 2024-01-20T02:02:06Z 2024-01-20T02:02:06Z 2021-09-05 2017-03 1838-7640 https://pubs.kist.re.kr/handle/201004/122980 Metabolic labeling techniques are powerful tools for cell labeling, tracking and proteomic analysis. However, at present, the effects of the metabolic labeling agents on cell metabolism and physiology are not known. To address this question, in this study, we analyzed the effects of cells treated with Ac4ManNAz through microarray analysis and analyses of membrane channel activity, individual bio-physiological properties, and glycolytic flux. According to the results, treatment with 50 mu M Ac4ManNAz led to the reduction of major cellular functions, including energy generation capacity, cellular infiltration ability and channel activity. Interestingly, 10 mu M Ac4ManNAz showed the least effect on cellular systems and had a sufficient labeling efficiency for cell labeling, tracking and proteomic analysis. Based on our results, we suggest 10 mu M as the optimum concentration of Ac4ManNAz for in vivo cell labeling and tracking. Additionally, we expect that our approach could be used for cell-based therapy for monitoring the efficacy of molecule delivery and the fate of recipient cells. English IVYSPRING INT PUBL CLICK CHEMISTRY QUANTITATIVE PROTEOMICS SIGNALING PATHWAYS N-GLYCOSYLATION LIVING ANIMALS AZIDO SUGARS THERAPY GLYCANS DEATH BIOCONJUGATION Physiological Effects of Ac4ManNAz and Optimization of Metabolic Labeling for Cell Tracking Article 10.7150/thno.17711 1 THERANOSTICS, v.7, no.5, pp.1164 - 1176 THERANOSTICS 7 5 1164 1176 scie scopus 000396574200010 2-s2.0-85014582959 Medicine, Research & Experimental Research & Experimental Medicine Article CLICK CHEMISTRY QUANTITATIVE PROTEOMICS SIGNALING PATHWAYS N-GLYCOSYLATION LIVING ANIMALS AZIDO SUGARS THERAPY GLYCANS DEATH BIOCONJUGATION Metabolic labeling Azido-sugar Modified glycosylation Azido group Physiology