Yoo, Junsang Chang, Yujung Kim, Hongwon Baek, Soonbong Choi, Hwan Jeong, Gun-Jae Shin, Jaein Kim, Hongnam Kim, Byung-Soo Kim, Jongpil 2024-01-20T02:02:12Z 2024-01-20T02:02:12Z 2021-09-01 2017-03 1550-7033 https://pubs.kist.re.kr/handle/201004/122986 Induced cardiomyocytes (iCMs) generated via direct lineage reprogramming offer a novel therapeutic target for the study and treatment of cardiac diseases. However, the efficiency of iCM generation is significantly low for therapeutic applications. Here, we show an efficient direct conversion of somatic fibroblasts into iCMs using nanotopographic cues. Compared with flat substrates, the direct conversion of fibroblasts into iCMs on nanopatterned substrates resulted in a dramatic increase in the reprogramming efficiency and maturation of iCM phenotypes. Additionally, enhanced reprogramming by substrate nanotopography was due to changes in the activation of focal adhesion kinase and specific histone modifications. Taken together, these results suggest that nanotopographic cues can serve as an efficient stimulant for direct lineage reprogramming into iCMs. English AMER SCIENTIFIC PUBLISHERS EMBRYONIC STEM-CELLS FOCAL ADHESION KINASE MOUSE FIBROBLASTS BIOPHYSICAL REGULATION EXTRACELLULAR-MATRIX DOPAMINERGIC-NEURONS CARDIAC FIBROBLASTS PLURIPOTENT STATE DEFINED FACTORS NUCLEAR SHAPE Efficient Direct Lineage Reprogramming of Fibroblasts into Induced Cardiomyocytes Using Nanotopographical Cues Article 10.1166/jbn.2017.2347 1 JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, v.13, no.3, pp.269 - 279 JOURNAL OF BIOMEDICAL NANOTECHNOLOGY 13 3 269 279 scie scopus 000397029400003 2-s2.0-85016037541 Nanoscience & Nanotechnology Materials Science, Biomaterials Science & Technology - Other Topics Materials Science Article EMBRYONIC STEM-CELLS FOCAL ADHESION KINASE MOUSE FIBROBLASTS BIOPHYSICAL REGULATION EXTRACELLULAR-MATRIX DOPAMINERGIC-NEURONS CARDIAC FIBROBLASTS PLURIPOTENT STATE DEFINED FACTORS NUCLEAR SHAPE Direct Lineage Reprogramming Induced Cardiomyocytes Nanotopographical Cues