Kim, Myungsuk Lee, Hee Ju Randy, Ahmad Yun, Ji Ho Oh, Sang-Rok Nho, Chu Won 2024-01-20T02:03:25Z 2024-01-20T02:03:25Z 2021-09-01 2017-02-21 2045-2322 https://pubs.kist.re.kr/handle/201004/123053 Stellera chamaejasme L. (Thymelaeaceae) is a perennial herb that is widely used in traditional Chinese medicine to treat tumours, tuberculosis and psoriasis. S. chamaejasme extract (SCE) possesses anti-inflammatory, analgesic and wound healing activities; however, the effect of S. chamaejasme and its active compounds on cutaneous wound healing has not been investigated. We assessed full-thickness wounds of Sprague-Dawley (SD) rats and topically applied SCE for 2 weeks. In vitro studies were performed using HaCaT keratinocytes, Hs68 dermal fibroblasts and RAW 264.7 macrophages to determine cell viability (MTT assay), cell migration, collagen expression, nitric oxide (NO) production, prostaglandin E-2 (PGE(2)) production, inflammatory cytokine expression and beta-catenin activation. In vivo, wound size was reduced and epithelisation was improved in SCE-treated SD rats. In vitro, SCE and its active compounds induced keratinocyte migration by regulating the beta-catenin, extracellular signal-regulated kinase and Akt signalling pathways. Furthermore, SCE and its active compounds increased mRNA expression of type I and III collagen in Hs68 fibroblasts. SCE and chamechromone inhibited NO and PGE2 release and mRNA expression of inflammatory mediators in RAW 264.7 macrophages. SCE enhances the motility of HaCaT keratinocytes and improves cutaneous wound healing in SD rats. English NATURE PUBLISHING GROUP NITRIC-OXIDE SYNTHASE GROWTH-FACTOR-BETA DEPENDENT TRANSCRIPTIONAL ACTIVATION CENTELLA-ASIATICA CATENIN STABILIZATION INSECTICIDAL ACTIVITY SKIN MACROPHAGES COLLAGEN CELLS Stellera chamaejasme and its constituents induce cutaneous wound healing and anti-inflammatory activities Article 10.1038/srep42490 1 SCIENTIFIC REPORTS, v.7 SCIENTIFIC REPORTS 7 scie scopus 000394709200001 2-s2.0-85013395127 Multidisciplinary Sciences Science & Technology - Other Topics Article NITRIC-OXIDE SYNTHASE GROWTH-FACTOR-BETA DEPENDENT TRANSCRIPTIONAL ACTIVATION CENTELLA-ASIATICA CATENIN STABILIZATION INSECTICIDAL ACTIVITY SKIN MACROPHAGES COLLAGEN CELLS Stellera chamaejasme wound healing cell migration collagen expression inflammatory cytokine