Cho, Yoon Lim, Jung-hee Song, Mi-Kyung Jeong, Seung-Chan Lee, Kyuhong Heo, Yongju Kim, Tae Sung Ryu, Jae-Chun 2024-01-20T02:04:30Z 2024-01-20T02:04:30Z 2021-09-01 2017-02 1520-4081 https://pubs.kist.re.kr/handle/201004/123112 Hexanal is a major component of indoor air pollutants and is a kind of aldehydes; it has adverse effects on human health. We performed an in vivo inhalation study and transcriptomic analysis to determine the mode of toxic actions in response to hexanal. Fischer 344 rats of both sexes were exposed by inhalation to hexanal aerosol for 4 hday(-1), 5 daysweek(-1) for 4 weeks at 0, 600, 1000, and 1500 ppm. Throughout our microarray-based genome-wide expression analysis, we identified 56 differentially expressed genes in three doses of hexanal; among these genes, 11 genes showed dose-dependent expression patterns (10 downregulated and 1 upregulated, 1.5-fold, p<0.05). Through a comparative toxicogenomics database (CTD) analysis of 11 genes, we determined that five genes (CCL12, DDIT4, KLF2, CEBPD, and ADH6) are linked to diverse disease categories such as cancer, respiratory tract disease, and immune system disease. These diseases were previously known for being induced by volatile organic compounds (VOCs). Our data demonstrated that the hexanal-induced dose-dependent altered genes could be valuable quantitative biomarkers to predict hexanal exposure and to perform relative risk assessments, including pulmonary toxicity. (c) 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 382-396, 2017. English WILEY-BLACKWELL INDOOR AIR-POLLUTION HUMAN ALVEOLAR CELLS GENE-EXPRESSION INHALATION TOXICITY PROINFLAMMATORY ACTIVATION FISCHER-344 RATS DRINKING-WATER DOSE-RESPONSE EXPOSURE LUNG Toxicogenomic analysis of the pulmonary toxic effects of hexanal in F344 rat Article 10.1002/tox.22242 1 ENVIRONMENTAL TOXICOLOGY, v.32, no.2, pp.382 - 396 ENVIRONMENTAL TOXICOLOGY 32 2 382 396 scie scopus 000393799500003 2-s2.0-84959423437 Environmental Sciences Toxicology Water Resources Environmental Sciences & Ecology Toxicology Water Resources Article INDOOR AIR-POLLUTION HUMAN ALVEOLAR CELLS GENE-EXPRESSION INHALATION TOXICITY PROINFLAMMATORY ACTIVATION FISCHER-344 RATS DRINKING-WATER DOSE-RESPONSE EXPOSURE LUNG indoor air pollutant aldehyde hexanal respiratory system environmental genomics