Kim, Eun J. Bond, Michelle R. Nam, Ghilsoo Hanover, John A. 2024-01-20T02:30:26Z 2024-01-20T02:30:26Z 2021-09-01 2017-02 0253-2964 https://pubs.kist.re.kr/handle/201004/123142 6-Azido-6-deoxy-N-acetylglucosamine (6AzGlcNAc) was recently introduced as a new selective metabolic chemical reporter (MCR) for labeling O-GlcNAc-modified proteins in cells. To investigate whether O-6AzGlcNAc is readily cleaved by O-GlcNAcase (OGA), the enzyme responsible for removing the GlcNAc modification, we synthesized PNP-6AzGlcNAc. This analog mimics O-GlcNAc and can be used in vitro to define the kinetic parameters for OGA thereby defining whether O-6AzGlcNAc can be employed as an appropriate tool to monitor O-GlcNAc dynamics in cells. Both PNP-6AzGlcNAc and PNP-GlcNAc were efficiently hydrolyzed by OGA with similar kinetics suggesting that an azidomodification at the GlcNAc C6 position does not significantly interfere with the beta-hexosaminidase activity of OGA. English 대한화학회 Evaluation of the Chemical Reporter Analog PNP-6AzGlcNAc as an O-GlcNAcase Substrate Article 10.1002/bkcs.11076 1 Bulletin of the Korean Chemical Society, v.38, no.2, pp.264 - 270 Bulletin of the Korean Chemical Society 38 2 264 270 N scie scopus kci ART002196127 000396907300017 2-s2.0-85010543342 Chemistry, Multidisciplinary Chemistry Article BETA-N-ACETYLGLUCOSAMINIDASE ALZHEIMERS-DISEASE MODIFIED PROTEINS LINKED GLCNAC GLCNACYLATION METABOLISM TAU PHOSPHORYLATION STRESS CELLS Metabolic chemical reporter O-GlcNAcase O-GlcNAc PNP-6AzGlcNAc