Kim, Dong-Woo Kwon, Jaeyoung Sim, Su Jin Lee, Dongho Mar, Woongchon 2024-01-20T02:30:36Z 2024-01-20T02:30:36Z 2021-09-01 2017-02 1756-4646 https://pubs.kist.re.kr/handle/201004/123152 We investigated the neuroprotective effects of orobol derivatives and ethanol extracts from Cudrania tricuspidata fruits. Among the nine isolates from a 50% ethanol extract from Cudrania tricuspidata fruits (CTE50), orobol (OB), 6-prenylorobol (POB), and 6,8-diprenylorobol (DPOB) showed neuroprotective effects in 6-OHDA-induced SH-SY5Y cell death. In addition, CTE50 and the three orobol derivatives (OB, POB, and DPOB) attenuated the cleavage of caspase-3, caspase-9, and PARP and inhibited the excessive generation of ROS. Furthermore, it enhanced the 6-OHDA-induced dysfunction of proteasome activity and reduced the accumulation of ubiquitin conjugated-proteins and the polyubiquitination of alpha-synuclein and synphilin-1. The proteasome inhibitor MG132 blocked the neuroprotective effects and the enhanced proteasome activity produced by CTE50 and the three orobol derivatives. These results demonstrate that CTE50 and three orobol derivatives protect against 6-OHDA-induced neurotoxicity by enhancing the ubiquitin/proteasome-dependent degradation of alpha-synuclein and synphilin-1, suggesting that they might be possible candidates for the treatment of neurodegenerative diseases. (C) 2016 Elsevier Ltd. All rights reserved. English ELSEVIER SCIENCE BV PARKINSONS-DISEASE OXIDATIVE STRESS ISOPRENYLATED XANTHONES SH-SY5Y CELLS ROOT BARK APOPTOSIS NEUROPROTECTION NEUROTOXICITY CONSTITUENTS ISOFLAVONES Orobol derivatives and extracts from Cudrania tricuspidata fruits protect against 6-hydroxydomamine-induced neuronal cell death by enhancing proteasome activity and the ubiquitin/proteasome-dependent degradation of alpha-synuclein and synphilin-1 Article 10.1016/j.jff.2016.12.017 1 JOURNAL OF FUNCTIONAL FOODS, v.29, pp.104 - 114 JOURNAL OF FUNCTIONAL FOODS 29 104 114 scie scopus 000393847800013 2-s2.0-85006792185 Food Science & Technology Nutrition & Dietetics Food Science & Technology Nutrition & Dietetics Article PARKINSONS-DISEASE OXIDATIVE STRESS ISOPRENYLATED XANTHONES SH-SY5Y CELLS ROOT BARK APOPTOSIS NEUROPROTECTION NEUROTOXICITY CONSTITUENTS ISOFLAVONES Cudrania tricuspidata Orobol derivatives Proteasome activity Polyubiquitination Neuroprotection 6-OHDA