Kim, Da Hye Kim, Myungsuk Oh, Sang-Bin Lee, Kyung Mi Kim, Sang Min Nho, Chu Won Yoon, Won Byong Kang, Kyungsu Pan, Cheol-Ho 2024-01-20T02:30:48Z 2024-01-20T02:30:48Z 2021-09-01 2017-02 0145-8884 https://pubs.kist.re.kr/handle/201004/123164 Herein, we prepared standardized antioxidant-enriched fractions from red (AFR) and purple (AFP) colored potatoes and evaluated their protective effects against hepatotoxicity induced by oxidative stress. The major compound contained in both AFR and AFP was 5-caffeoylquinic acid. Experiments in vitro showed that AFR and AFP protected against HepG2 cell death induced by tert-butyl hydroperoxide (t-BHP) as determined by cell viability assay. AFR and AFP recovered alanine aminotransferase (ALT) and aspartate aminotransferase (AST) enzyme activities, which increased by t-BHP treatment in HepG2 cells. AFR and AFP also inhibited the cleavage of PARP and caspase-3 in HepG2 cells. Moreover, in vivo experiments in mice showed that AFR and AFP protected against t-BHP-induced hepatotoxicity and recovered serum ALT and AST enzyme activities. AFR and AFP also rescued serum antioxidant enzyme activities, catalase and glutathione peroxidase, which were disturbed by t-BHP treatment. Our data clearly demonstrate that AFR and AFP protect against hepatic damage induced by oxidative stress. English WILEY PURPLE POTATO HYDROXYCINNAMIC ACIDS ANTHOCYANINS EXTRACT DAMAGE LIVER APOPTOSIS PHENOLICS DIGESTION CANCER THE PROTECTIVE EFFECT OF ANTIOXIDANT ENRICHED FRACTIONS FROM COLORED POTATOES AGAINST HEPATOTOXIC OXIDATIVE STRESS IN CULTURED HEPATOCYTES AND MICE Article 10.1111/jfbc.12315 1 JOURNAL OF FOOD BIOCHEMISTRY, v.41, no.1 JOURNAL OF FOOD BIOCHEMISTRY 41 1 scie scopus 000397284000016 2-s2.0-84982883755 Biochemistry & Molecular Biology Food Science & Technology Biochemistry & Molecular Biology Food Science & Technology Article PURPLE POTATO HYDROXYCINNAMIC ACIDS ANTHOCYANINS EXTRACT DAMAGE LIVER APOPTOSIS PHENOLICS DIGESTION CANCER colored potato antioxidant hepatoprotective anthocyanin hydroxycinnamate HepG2