Oh, Eun Hae Jung, Seungwon Kim, Won Jin Kim, Kwang Pyo Kim, Sang Kyung 2024-01-20T02:31:23Z 2024-01-20T02:31:23Z 2021-09-05 2017-01-15 0956-5663 https://pubs.kist.re.kr/handle/201004/123192 The quantitative reverse transcription polymerase chain reaction (RT-qPCR) has become one of the most widely used methods in the detection of disease-specific RNAs. The RT-qPCR involves two separate steps, RT and qPCR. In this study, we suggest a new RT-qPCR protocol with the particles of primer-immobilized networks (PINs), performing capture, RT and amplification of a target RNA in one particle. The production of undesired cDNAs was dramatically suppressed by the specific capture of the target RNA within the particle. Afterward, RT and amplification processes are performed without loss of cDNAs as exchanging the reaction solution. The biomarker gene of chronic myeloid leukemia, Bcr-Abl fusion transcript, is detected in the sensitivity of single mutant leukemic cell mixed in 104 normal cell using this protocol with the excellent restraint of non-specific signal. This protocol that whole processes are performed in the particle in a row is preferred for the highly specific detection of target RNAs in complex sample. (C) 2016 Elsevier B.V. All rights reserved. English ELSEVIER ADVANCED TECHNOLOGY REAL-TIME PCR POLYMERASE-CHAIN-REACTION SINGLE-CELL DIAGNOSIS INFECTIONS CANCER ASSAY GENE AMPLIFICATION HYBRIDIZATION Microparticle-based RT-qPCR for highly selective rare mutation detection Article 10.1016/j.bios.2016.08.057 1 BIOSENSORS & BIOELECTRONICS, v.87, pp.229 - 235 BIOSENSORS & BIOELECTRONICS 87 229 235 scie scopus 000390499600034 2-s2.0-84989819446 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article REAL-TIME PCR POLYMERASE-CHAIN-REACTION SINGLE-CELL DIAGNOSIS INFECTIONS CANCER ASSAY GENE AMPLIFICATION HYBRIDIZATION RT-qPCR Primer-immobilized particles Bcr-Abl fusion transcript