Suhaeri, Muhammad Subbiah, Ramesh Kim, Su-Hyun Kim, Chong-Hyun Oh, Seung Ja Kim, Sang-Heon Park, Kwideok 2024-01-20T02:31:39Z 2024-01-20T02:31:39Z 2021-09-05 2017-01-11 1944-8244 https://pubs.kist.re.kr/handle/201004/123207 For cardiac tissue engineering, much attention has been given to the artificial cardiac microenvironment in winch anisotropic design of scaffold and extracellular matrix (ECM) are the major cues. Here we propose poly(L-lactide-co-caprolactone) and fibroblast-derived ECM (PLCL/FDM), a hybrid scaffold that combines aligned electrospun PLCL fibers and FDM. Fibroblasts were grown on the PLCL fibers for 5 7 days and subsequently decellularized to produce PLCL/FDM. Various analyses confirmed aligned, FDM-deposited PLCL fibers. Compared to fibronectin (FN)-coated electrospun PLCL fibers (control), H9c2 cardiomyoblast differentiation was significantly effective, and neonatal rat cardiomyocyte (CM) phenotype and maturation was improved on PLCL/FDM. Moreover, a coculture platform was created using multilayer PLCL/FDM in which two different cells make indirect or direct cell cell contacts. Such coculture platforms demonstrate their feasibility in terms of higher cell viability, efficiency of target cell harvest (>95% in noncontact; 85% in contact mode), and molecular diffusion through the PLCL/FDM layer. Coculture of primary CMs and fibroblasts exhibited much better CM phenotype and improvement of CM maturity upon either direct or indirect interactions, compared to the conventional coculture systems (transwell insert and tissue culture plate (TCP)). Taken together, our platform should be very useful and have significant contributions in investigating some scientific or practical issues of crosstalks between multiple cell types. English American Chemical Society CHRONIC HEART-FAILURE EXTRACELLULAR-MATRIX IN-VITRO DIFFERENTIATED PHENOTYPE FUNCTIONAL MATURATION MYOCARDIAL-INFARCTION CARDIAC MICROTISSUES STROMAL CELLS H9C2 CELLS PROLIFERATION Novel Platform of Cardiomyocyte Culture and Coculture via Fibroblast-Derived Matrix-Coupled Aligned Electrospun Nanofiber Article 10.1021/acsami.6b14020 1 ACS Applied Materials & Interfaces, v.9, no.1, pp.224 - 235 ACS Applied Materials & Interfaces 9 1 224 235 scie scopus 000392037400029 Nanoscience & Nanotechnology Materials Science, Multidisciplinary Science & Technology - Other Topics Materials Science Article CHRONIC HEART-FAILURE EXTRACELLULAR-MATRIX IN-VITRO DIFFERENTIATED PHENOTYPE FUNCTIONAL MATURATION MYOCARDIAL-INFARCTION CARDIAC MICROTISSUES STROMAL CELLS H9C2 CELLS PROLIFERATION cardiac tissue engineering fibroblast-derived ECM coculture platform H9c2 cardiomyoblast neonatal rat cardiomyocytes