Ryu, Seung Mok Kwon, Jaeyoung Seo, Young Hye Song, Eun Gyeong Hong, Seong Su Kim, Beom Seok Hong, Jin Sung Ryu, Ki Hyun Lee, Dongho 2024-01-20T02:31:54Z 2024-01-20T02:31:54Z 2021-09-05 2017-01-02 0168-1702 https://pubs.kist.re.kr/handle/201004/123220 A green fluorescent protein (GFP)-tagged pepper mottle virus (PepMoV) based leaf-disc method and systemic host method were developed to identify antiviral agents. Preliminary experiments using a PepMoV-GFP based leaf-disc method led to the isolation of five quassinoids, including brusatol (1), bruceantin (2), brucein A (3), bruceantinol (4), and brucein B (5), from the CH3OH extract of Brucea javanica. All isolated compounds exhibited inactivation effects in systemic host plants, and compounds 3 and 4 were potent, with a minimum inhibitory concentration of 10 M. Furthermore, compound 3 was found to have a protective effect at the tested concentration of 40 M. (C) 2016 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV MOSAIC-VIRUS STRUCTURAL ELUCIDATION AGENTS SEEDS Quassinoids isolated from Brucea javanica inhibit pepper mottle virus in pepper Article 10.1016/j.virusres.2016.09.013 1 VIRUS RESEARCH, v.227, pp.49 - 56 VIRUS RESEARCH 227 49 56 scie scopus 000389729700007 2-s2.0-84991830752 Virology Virology Article MOSAIC-VIRUS STRUCTURAL ELUCIDATION AGENTS SEEDS Pepper mottle virus Green fluorescent protein Capsicum annuum Brucea javanica Quassinoid Antiviral agent