Wu, Wenming Manz, Andreas 2024-01-20T02:32:05Z 2024-01-20T02:32:05Z 2022-01-25 2017-01 2046-2069 https://pubs.kist.re.kr/handle/201004/123230 Herein, we introduce a practical and effective manufacturing methodology for a biomimetic microdevice replicated from the Tilia platyphyllos leaf. With this method, artificial microchambers (of controllable dimension and depth) can be easily integrated into leaf-inspired whole-ordered venation patterns. To display the biocompatibility of this microdevice, we applied it to a long-term (seven days) cell culture and monitored the results. Based on a comprehensive biophysical analysis, including covering cellular deformation, cell migration, cytomembrane tension, extracellular communication, protonema formation, microvilli, and the tethers' dynamic of human melanoma cells inside the device at a single-cell resolution, we were able to verify for the first time a leaf-inspired PDMS microdevice as a biocompatible platform for mammal cell culture, showing promise that such a biomimetic device could be further applied for organ-on-a-chip studies and other biomedical research. English ROYAL SOC CHEMISTRY Biocompatibility assay of cellular behavior inside a leaf-inspired biomimetic microdevice at the single-cell level Article 10.1039/c7ra00290d 1 RSC ADVANCES, v.7, no.52, pp.32710 - 32720 RSC ADVANCES 7 52 32710 32720 N scie scopus 000404609800034 Chemistry, Multidisciplinary Chemistry Article POLYMERASE-CHAIN-REACTION ORGANS-ON-CHIPS MICROFLUIDIC PLATFORM DRUG DISCOVERY MURRAYS LAW REAL-TIME A-CHIP MIGRATION SYSTEM MICROSYSTEM