Jang, Yura Je, Young Tae Yun, Cheol-Won Chung, Hesson 2024-01-20T02:32:11Z 2024-01-20T02:32:11Z 2021-09-05 2017-01 0141-8130 https://pubs.kist.re.kr/handle/201004/123236 This study was designed to investigate the oil entrapment and systemic oil absorption-reducing activities of chitosan. High-molecular-weight chitosan formed gel aggregates with oil and bile salts in vitro. The oil/chitosan ratio and the molecular weight of chitosan were optimized for the in vivo study, and a molecular weight >100,000 was effective in reducing the oil contamination of mouse fur. The oil/chitosan weight ratio required for effective oil entrapment was less than 13 and 5 in the in vitro and in vivo experiments, respectively. Chitosan administration was most effective during meals, and high-molecular-weight chitosan could trap and facilitate the reduction of systemic absorption of oil droplets separated by orlistat. The activity of the lipase inhibitor was not altered by chitosan as evidenced by thin layer chromatography, and orlistat was not absorbed systemically by the co-administration of chitosan. (C) 2016 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV HIGH-FAT DIET MOLECULAR-WEIGHT CHITOSAN BODY-WEIGHT LONG-TERM RATS SUPPLEMENTATION ORLISTAT CHITIN OVERWEIGHT CHOLESTEROL Chitosan dosage regimen to trap fecal oil excretion after peroral lipase inhibitor administration in mice Article 10.1016/j.ijbiomac.2016.10.003 1 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, v.94, pp.484 - 491 INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 94 484 491 scie scopus 000390621900054 2-s2.0-84992378722 Biochemistry & Molecular Biology Chemistry, Applied Polymer Science Biochemistry & Molecular Biology Chemistry Polymer Science Article HIGH-FAT DIET MOLECULAR-WEIGHT CHITOSAN BODY-WEIGHT LONG-TERM RATS SUPPLEMENTATION ORLISTAT CHITIN OVERWEIGHT CHOLESTEROL Chitosan Oil entrapment Lipase inhibitor