Kim, Sang Jun Lee, Sang Mok Kim, Ji Eun Kim, Soo Hyun Jung, Youngmee 2024-01-20T02:32:34Z 2024-01-20T02:32:34Z 2021-09-05 2017-01 1932-6254 https://pubs.kist.re.kr/handle/201004/123257 Several trials have been carried out to improve the healing of rotator cuff tear, but their effects remain controversial. In this study, we examined the therapeutic effect of platelet-rich plasma (PRP) in combination with self-assembled peptide (SAP) on the healing of rotator cuff tear in the rat. Twenty-seven Sprague-Dawley rats, aged 15 weeks, were used for the rotator cuff tear model. The supraspinatus muscle at the insertion site was dissected and truncated, leaving the cut edge free. A week after the surgery, the rats were randomly divided into SAP, PRP, SAP-PRP and control groups and 0.2 ml SAP, PRP, SAP-PRP and saline were injected, respectively. Bonar scores and synovial inflammation grade were checked 5 weeks after the injection. Immunofluorescence staining for heat shock protein (HSP)-70 and caspase-3 was conducted. Furthermore, maximum stride length was measured before and at 2 and 4 weeks after the injection. The Bonar scores were 6.4 +/- 1.8 in the SAP group, 5.9 +/- 2.0 in the PRP group, 4.7 +/- 1.1 in the SAP-PRP group and 8.3 +/- 2.3 in the control group. There was significant difference between the SAP-PRP and control groups in post hoc multiple comparison analysis. Among four categories of Bonar scores, collagen arrangement and vascular infiltration showed improvement after SAP-PRP injection. Immunofluorescence images showed that HSP-70 and caspase-3 were much less expressed in the SAP-PRP group than in the other groups. In conclusion, SAP-PRP can be effective in healing a rotator cuff tear by enhancing the collagen arrangement and inhibiting inflammatory changes and apoptosis. Copyright (c) 2015 John Wiley & Sons, Ltd. English WILEY MESENCHYMAL STEM-CELLS IN-VIVO CARDIAC-FUNCTION GENE-EXPRESSION REPAIR GROWTH TENDONS SUPRASPINATUS PROLIFERATION INJECTIONS Effect of platelet-rich plasma with self-assembled peptide on the rotator cuff tear model in rat Article 10.1002/term.1984 1 JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v.11, no.1, pp.77 - 85 JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE 11 1 77 85 scie scopus 000394173600007 2-s2.0-84921728748 Cell & Tissue Engineering Biotechnology & Applied Microbiology Cell Biology Engineering, Biomedical Cell Biology Biotechnology & Applied Microbiology Engineering Article MESENCHYMAL STEM-CELLS IN-VIVO CARDIAC-FUNCTION GENE-EXPRESSION REPAIR GROWTH TENDONS SUPRASPINATUS PROLIFERATION INJECTIONS rotator cuff tear self-assembled peptide platelet-rich plasma tendon regeneration anti-inflammation apoptosis