Lee, Kyeong Won Yim, Hyung-Soon Shin, Jihye Lee, Cheolju Lee, Jung-Hyun Jeong, Jae-Yeon 2024-01-20T02:32:49Z 2024-01-20T02:32:49Z 2021-09-04 2017-01 0014-5793 https://pubs.kist.re.kr/handle/201004/123272 Fibroblast growth factor 11 (FGF11) is an intracellular FGF. Although induction of FGF11 by hypoxia has been observed in several cell types, the molecular function of FGF11 is not clearly understood yet. Here, we investigated the role of FGF11 under hypoxia. We identified hypoxia-inducible factor-1 alpha (HIF-1 alpha) as an interacting protein of FGF11 using immunoprecipitation and mass spectrometry. FGF11 knockdown decreased HIF-1 alpha protein, while FGF11 overexpression increased it, without affecting HIF-1 alpha mRNA. Protein stability test and ubiquitination assay showed that FGF11 increased HIF-1 alpha stability by acting upstream of proteasomal degradation. Altogether, these results suggest a cross-regulation between HIF-1a and FGF11, through which hypoxia-induced FGF11 reinforces hypoxia responses by enhancing the stability of HIF-1 alpha. English WILEY FACTOR HOMOLOGOUS FACTORS INDUCIBLE FACTORS EXPRESSION CANCER CELLS INDUCTION EVOLUTION THERAPY PROTEIN FGF11 induced by hypoxia interacts with HIF-1 alpha and enhances its stability Article 10.1002/1873-3468.12547 1 FEBS LETTERS, v.591, no.2, pp.348 - 357 FEBS LETTERS 591 2 348 357 scie scopus 000393962400012 2-s2.0-85010510332 Biochemistry & Molecular Biology Biophysics Cell Biology Biochemistry & Molecular Biology Biophysics Cell Biology Article FACTOR HOMOLOGOUS FACTORS INDUCIBLE FACTORS EXPRESSION CANCER CELLS INDUCTION EVOLUTION THERAPY PROTEIN FGF11 HIF-1 alpha hypoxia intracellular fibroblast growth factor