Kim, Hong Nam Jang, Kyung-Jin Shin, Jung-Youn Kang, Daeshik Kim, Sang Moon Koh, Ilkyoo Hong, Yoonmi Jang, Segeun Kim, Mm Sung Kim, Byung-Soo Jeong, Hoon Eui Jeon, Noo Li Kim, Pilnam Suh, Kahp-Yang 2024-01-20T02:33:01Z 2024-01-20T02:33:01Z 2021-09-04 2017-01 1936-0851 https://pubs.kist.re.kr/handle/201004/123283 We present a method to induce cell directional behavior using slanted nanocilia arrays. NIH-3T3 fibroblasts demonstrated bidirectional polarization in a rectangular arrangement on vertical nanocilia arrays and exhibited a transition from a bidirectional to a unidirectional polarization pattern when the angle of the nanocilia was decreased from 90 degrees to 30 degrees. The slanted nanocilia guided and facilitated spreading by allowing the cells to contact the sidewalls of the nanocilia, and the directional migration of the cells opposed the direction of the slant due to the anisotropic bending stiffness of the slanted nanocilia. Although the cells recognized the underlying anisotropic geometry when the nanocilia were coated with fibronectin, collagen type I, and Matrigel, the cells lost their directionality when the nanocilia were coated with poly-D-lysine and poly-L-lysine. Furthermore, although the cells recognized geometrical anisotropy on fibronectin coatings, pharmacological perturbation of PI3K-Rac signaling hindered the directional elongation of the cells on both the slanted and vertical nanocilia. Furthermore, myosin light chain II was required for the cells to obtain polarized morphologies. These results indicated that the slanted nanocilia array provided anisotropic contact guidance cues to the interacting cells. The polarization of cells was controlled through two steps: the recognition of underlying geometrical anisotropy and the subsequent directional spreading according to the guidance cues. English AMER CHEMICAL SOC NEURAL STEM-CELLS FOCAL ADHESION KINASE CONTACT GUIDANCE NEURONAL DIFFERENTIATION EXTRACELLULAR-MATRIX NANOTOPOGRAPHY CILIA MIGRATION TOPOGRAPHY SUBSTRATE Artificial Slanted Nanocilia Array as a Mechanotransducer for Controlling Cell Polarity Article 10.1021/acsnano.6b07134 1 ACS NANO, v.11, no.1, pp.730 - 741 ACS NANO 11 1 730 741 scie scopus 000392886500075 2-s2.0-85018499681 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science Article NEURAL STEM-CELLS FOCAL ADHESION KINASE CONTACT GUIDANCE NEURONAL DIFFERENTIATION EXTRACELLULAR-MATRIX NANOTOPOGRAPHY CILIA MIGRATION TOPOGRAPHY SUBSTRATE nanocilia slanted angle anisotropy polarization unidirectional