Van-Thuan Nguyen Seo, Ho Bin Kim, Byoung Chan Kim, Sang Kyung Song, Chang-Seon Gu, Man Bock 2024-01-20T02:33:39Z 2024-01-20T02:33:39Z 2021-09-04 2016-12-15 0956-5663 https://pubs.kist.re.kr/handle/201004/123316 In this research, we report highly sensitive and specific sandwich-type SPR-based biosensor for the detection H5Nx whole viruses. A few of aptamers, for the first time, were successfully screened and characterized for whole avian influenza (AI) viruses, H5Nx, by using Multi-GO-SELEX method. The affinities of the aptamers developed in this study were ranged from 8 x 10(4) to 1 x 10(4) EID50/ml, and the aptamers IF22, IF23 were found to be specific to H5N1 and H5N8, respectively. In addition, some flexible aptamers IF20, IF15, and IF10 were found to bind to the H5N1 and H5N2, H5N1 and H5N8, or H5N1, H5N2, and H5N8, respectively. Moreover, aptamers IF10 and IF22 were found to bind H5N1 virus simultaneously and confirmed to bind the different site of the same H5N1 whole virus. Therefore, this pair of aptamers, IF10 and IF22, were successfully applied to develop the sandwich-type SPR-based biosensor assay which is rapid, accurate for the detection of Al whole virus from H5N1-infected feces samples. The minimum detectible concentration of H5N1 whole virus was found to be 200 EID50/ml with this sandwich-type detection using the aptamer pair obtained in this study. In addition, the sensitivity of this biosensor was successfully enhanced by using the signal amplification with the secondary aptamer conjugated with gold nanoparticles. (C) 2016 Elsevier B.V. All rights reserved. English ELSEVIER ADVANCED TECHNOLOGY AVIAN INFLUENZA-VIRUS RECEPTOR-BINDING REGION DNA APTAMERS RNA APTAMER MONOCLONAL-ANTIBODY HEMAGGLUTININ INFECTION SELECTION ELISA NANOPARTICLES Highly sensitive sandwich-type SPR based detection of whole H5Nx viruses using a pair of aptamers Article 10.1016/j.bios.2016.06.064 1 BIOSENSORS & BIOELECTRONICS, v.86, pp.293 - 300 BIOSENSORS & BIOELECTRONICS 86 293 300 scie scopus 000384853300041 2-s2.0-84978967803 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article AVIAN INFLUENZA-VIRUS RECEPTOR-BINDING REGION DNA APTAMERS RNA APTAMER MONOCLONAL-ANTIBODY HEMAGGLUTININ INFECTION SELECTION ELISA NANOPARTICLES Whole virus H5Nx Dual aptamer SPR AuNP Circular Dichroism