Lee, Su Hyeon Lee, Nanhee Hong, Youngmin Chung, Bong Chul Choi, Man Ho 2024-01-20T02:33:50Z 2024-01-20T02:33:50Z 2021-09-04 2016-12-06 0003-2700 https://pubs.kist.re.kr/handle/201004/123325 Sulfated steroids can act as a latent form of active free steroids, coexisting with them in biological specimens. To evaluate the metabolic significance of free and sulfated steroid species, a simultaneous analysis of eight free steroids [cholesterol, pregnenolone, 17 alpha-hydroxypregnenolone, progesterone, 17 alpha-hydroxyprogesterone, dehydroepiandrosterone (DHEA), testosterone, and 17 beta-estradiol] and four biologically relevant sulfated steroids was developed and validated, using selected-ion and multiple-reaction monitoring modes coupled to polarity-switching liquid chromatography/mass spectrometry (LC/MS). All steroids were separated on a reversed-phase phenyl column (50 mm X 2 mm, 3 mu m) at a flow rate of 0.5 mL/min. The limits of quantification ranged from 0.1 to 50 ng/mL at extraction recoveries of 94.1-105.5%, while the precision and accuracy were 2.5-9.3% and 92.4-105.9%, respectively. Quantitative results obtained for samples from obese girls showed that the serum levels of DHEA sulfate were significantly increased (P = 0.004), along with the metabolic ratio representing DHEA sulfotransferase (P < 0.02). The developed novel LC/MS method can quantitatively profile both free and sulfated steroids in a single analytical run. English AMER CHEMICAL SOC DEHYDROEPIANDROSTERONE-SULFATE LC-MS/MS HUMAN SERUM BODY-FAT CHOLESTEROL OBESITY WEIGHT SEX CHILDREN HORMONES Simultaneous Analysis of Free and Sulfated Steroids by Liquid Chromatography/Mass Spectrometry with Selective Mass Spectrometric Scan Modes and Polarity Switching Article 10.1021/acs.analchem.6b03183 1 ANALYTICAL CHEMISTRY, v.88, no.23, pp.11624 - 11630 ANALYTICAL CHEMISTRY 88 23 11624 11630 scie scopus 000389556900053 2-s2.0-85030691436 Chemistry, Analytical Chemistry Article DEHYDROEPIANDROSTERONE-SULFATE LC-MS/MS HUMAN SERUM BODY-FAT CHOLESTEROL OBESITY WEIGHT SEX CHILDREN HORMONES steroid sulfate mass spectrometry reproductive steroid obesity