Han, Hee Dong Byeon, Yeongseon Jang, Jong-Hwa Jeon, Hat Nim Kim, Ga Hee Kim, Min Gi Pack, Chan-Gi Kang, Tae Heung Jung, In Duk Lim, Yong Taik Lee, Young Joo Lee, Jeong-Won Shin, Byung Cheol Ahn, Hyung Jun Sood, Anil K. Park, Yeong-Min 2024-01-20T02:33:53Z 2024-01-20T02:33:53Z 2021-09-04 2016-12-02 2045-2322 https://pubs.kist.re.kr/handle/201004/123328 Dentritic cell (DC)-based cancer immunotherapy faces challenges in both efficacy and practicality. However, DC-based vaccination requires multiple injections and elaborates ex vivo manipulation, which substantially limits their use. Therefore, we sought to develop a chitosan nanoparticle (CH-NP)-based platform for the next generation of vaccines to bypass the ex vivo manipulation and induce immune responses via active delivery of polyinosinic-polycytidylic acid sodium salt (poly I: C) to target Toll-like receptor 3 (TLR3) in endosomes. We developed CH-NPs encapsulating ovalbumin (OVA) as a model antigen and poly I: C as the adjuvant in an ionic complex. These CH-NPs showed increased in vivo intracellular delivery to the DCs in comparison with controls after injection into tumor-bearing mice, and promoted DC maturation, leading to emergence of antigen-specific cytotoxic CD8+ T cells. Finally, the CH-NPs showed significantly greater antitumor efficacy in EG.7 and TC-1 tumor-bearing mice compared to the control (p < 0.01). Taken together, these data show that the CH-NP platform can be used as an immune response modulatory vaccine for active cancer immunotherapy without ex vivo manipulation, thus resulting in increased anticancer efficacy. English NATURE PUBLISHING GROUP CELL-BASED VACCINES DENDRITIC CELLS ANTITUMOR IMMUNITY TUMOR-CELLS DELIVERY TRACKING STIMULATION VACCINATION TOXICITY HYDROGEL In vivo stepwise immunomodulation using chitosan nanoparticles as a platform nanotechnology for cancer immunotherapy Article 10.1038/srep38348 1 SCIENTIFIC REPORTS, v.6 SCIENTIFIC REPORTS 6 scie scopus 000389084400003 2-s2.0-85003955264 Multidisciplinary Sciences Science & Technology - Other Topics Article CELL-BASED VACCINES DENDRITIC CELLS ANTITUMOR IMMUNITY TUMOR-CELLS DELIVERY TRACKING STIMULATION VACCINATION TOXICITY HYDROGEL 항암면역치료 나노파티클 난소암