Kim, Jiae Kim, Su-Min Na, Jung-Min Hahn, Hoh-Gyu Cho, Sung-Woo Yang, Seung-Ju 2024-01-20T02:34:03Z 2024-01-20T02:34:03Z 2022-01-25 2016-12 1976-6696 https://pubs.kist.re.kr/handle/201004/123338 We recently reported the anti-inflammatory effects of 3-(naphthalen-2-yl(propoxy) methyl) azetidine hydrochloride (KHG26792) on the ATP-induced activation of the NFAT and MAPK pathways through the P2X7 receptor in microglia. To further investigate the underlying mechanism of KHG26792, we studied its protective effects on hypoxia-induced toxicity in microglia. The administration of KHG26792 significantly reduced the hypoxia-induced expression and activity of caspase-3 in BV-2 microglial cells. KHG26792 also reduced hypoxia-induced inducible nitric oxide synthase protein expression, which correlated with reduced nitric oxide accumulation. In addition, KHG26792 attenuated hypoxia-induced protein nitration, reactive oxygen species production, and NADPH oxidase activity. These effects were accompanied by the suppression of hypoxia-induced protein expression of hypoxia-inducible factor 1-alpha and NADPH oxidase-2. Although the clinical relevance of our findings remains to be determined, these data results suggest that KHG26792 prevents hypoxia-induced toxicity by suppressing microglial activation. English KOREAN SOCIETY BIOCHEMISTRY & MOLECULAR BIOLOGY Protective effect of 3-(naphthalen-2-yl(propoxy)methyl) azetidine hydrochloride on hypoxia-induced toxicity by suppressing microglial activation in BV-2 cells Article 10.5483/BMBRep.2016.49.12.169 1 BMB REPORTS, v.49, no.12, pp.687 - 692 BMB REPORTS 49 12 687 692 N scie scopus kci ART002177126 000392180000007 2-s2.0-85009168533 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article TRIPLE REUPTAKE INHIBITORS BRAIN NEUROTOXICITY EXPLORATION DERIVATIVES PATHWAYS RECEPTOR DISEASE STRESS Hypoxia Microglia Oxidative stress 3-(Naphthalen-2-yl(propoxy)methyl)azetidine hydrochloride