Seonwoo, Hoon Bae, Won-Gyu Park, Sunho Kim, Hong-Nam Choi, Kyoung Soon Lim, Ki Taek Hyun, Hoon Kim, Jin-Woo Kim, Jangho Chung, Jong Hoon 2024-01-20T02:34:14Z 2024-01-20T02:34:14Z 2021-09-05 2016-12 1536-1241 https://pubs.kist.re.kr/handle/201004/123349 Living cells receive biochemical and physical information from the surrounding microenvironment and respond to this information. Multiscale hierarchical substrates with micro-and nanogrooves have been shown to mimic the native extracellular matrix (ECM) better than conventional nanopatterned substrates; therefore, substrates with hierarchical topographical cues are considered suitable for investigating the role of physical factors in tissue functions. In this study, precisely controllable, multiscale hierarchical substrates that could mimic the micro-and nanotopography of complex ECMs were fabricated and used to culture various cell types, including fibroblasts, endothelial cells, osteoblasts, and human mesenchymal stem cells. These substrates had both microscale wrinkles and nanoscale patterns and enhanced the alignment and elongation of all the cells tested. In particular, the nanotopography on the microscale wrinkles promoted not only the adhesion, but also the functions of the cells. These findings suggest that the hierarchical multiscale substrates effectively regulated cellular structure and functions and that they can be used as a platform for tissue engineering and regenerative medicine. English IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC MESENCHYMAL STEM-CELLS EXTRACELLULAR-MATRIX ELASTOMERIC POLYMER NANOTOPOGRAPHY CONSTRUCTS WOUNDS FATE Hierarchically Micro- and Nanopatterned Topographical Cues for Modulation of Cellular Structure and Function Article 10.1109/TNB.2016.2631641 1 IEEE TRANSACTIONS ON NANOBIOSCIENCE, v.15, no.8, pp.835 - 842 IEEE TRANSACTIONS ON NANOBIOSCIENCE 15 8 835 842 scie scopus 000394111400007 2-s2.0-85010403569 Biochemical Research Methods Nanoscience & Nanotechnology Biochemistry & Molecular Biology Science & Technology - Other Topics Article MESENCHYMAL STEM-CELLS EXTRACELLULAR-MATRIX ELASTOMERIC POLYMER NANOTOPOGRAPHY CONSTRUCTS WOUNDS FATE alignment cell elongation fibroblasts hierarchical structure mesenchymal stem cells