Cho, Yoon Song, Mi-Kyung Jeong, Seung-Chan Lee, Kyuhong Heo, Yongju Kim, Tae Sung Ryu, Jae-Chun 2024-01-20T02:34:30Z 2024-01-20T02:34:30Z 2021-09-05 2016-12 1520-4081 https://pubs.kist.re.kr/handle/201004/123364 In previous studies, we have investigated the relationships between environmental chemicals and health risk based on omics analysis and identified significant biomarkers. Our current findings indicate that hexanal may be an important toxicant of the pulmonary system in epigenetic insights. MicroRNA (miRNA) is an important indicator of biomedical risk assessment and target identification. Hexanal is highly detectable in the exhaled breath of patients with chronic obstructive pulmonary disease (COPD) and chronic inflammatory lung disease. In this study, we aimed to identify hexanal-characterized miRNA-mRNA correlations involved in lung toxicity. Microarray analysis identified 56 miRNAs that commonly changed their expression more than 1.3-fold in three doses (600, 1000, and 1500 ppm) within hexanal-exposed Fischer 344 rats by inhalation, and 226 genes were predicted to be target genes of miRNAs through TargetScan analysis. By integrating analyses of miRNA and mRNA expression profiles, we identified one anti-correlated target gene (Chga; chromogranin A; parathyroid secretory protein 1). Comparative toxicogenomics database (CTD) analysis of this gene showed that Chga is involved with several disease categories such as cancer, respiratory tract disease, nervous system disease, and cardiovascular disease. Further research is necessary to elucidate the mechanisms of hexanal-responsive toxicologic pathways at the molecular level. This study concludes that our integrated approach to miRNA and mRNA enables us to identify molecular events in disease development induced by hexanal in an in vivo rat model. (C) 2015 Wiley Periodicals, Inc. English WILEY POLYCYCLIC AROMATIC-HYDROCARBONS GENE-ENVIRONMENT INTERACTIONS CHROMOGRANIN-A DOSE-RESPONSE AIR-POLLUTION DISEASE EXPRESSION ALDEHYDES INDOOR IDENTIFICATION MicroRNA Response of Inhalation Exposure to Hexanal in Lung Tissues from Fischer 344 Rats Article 10.1002/tox.22192 1 ENVIRONMENTAL TOXICOLOGY, v.31, no.12, pp.1909 - 1921 ENVIRONMENTAL TOXICOLOGY 31 12 1909 1921 scie scopus 000388617700021 2-s2.0-84945371019 Environmental Sciences Toxicology Water Resources Environmental Sciences & Ecology Toxicology Water Resources Article POLYCYCLIC AROMATIC-HYDROCARBONS GENE-ENVIRONMENT INTERACTIONS CHROMOGRANIN-A DOSE-RESPONSE AIR-POLLUTION DISEASE EXPRESSION ALDEHYDES INDOOR IDENTIFICATION hexanal microRNA Fischer 344 rat lung Chga