Ko, Ja Kyong Um, Youngsoon Lee, Sun-Mi 2024-01-20T03:00:16Z 2024-01-20T03:00:16Z 2021-09-05 2016-12 0960-8524 https://pubs.kist.re.kr/handle/201004/123377 The efficient fermentation of lignocellulosic hydrolysates in the presence of inhibitors is highly desirable for bioethanol production. Among the inhibitors, acetic acid released during the pretreatment of lignocellulose negatively affects the fermentation performance of biofuel producing organisms. In this study, we evaluated the inhibitory effects of acetic acid on glucose and xylose fermentation by a high performance engineered strain of xylose utilizing Saccharomyces cerevisiae, SXA-R2P-E, harboring a xylose isomerase based pathway. The presence of acetic acid severely decreased the xylose fermentation performance of this strain. However, the acetic acid stress was alleviated by metal ion supplementation resulting in a 52% increased ethanol production rate under 2 g/L of acetic acid stress. This study shows the inhibitory effect of acetic acid on an engineered isomerase-based xylose utilizing strain and suggests a simple but effective method to improve the co-fermentation performance under acetic acid stress for efficient bioethanol production. (C) 2016 Elsevier Ltd. All rights reserved. English Elsevier BV Effect of manganese ions on ethanol fermentation by xylose isomerase expressing Saccharomyces cerevisiae under acetic acid stress Article 10.1016/j.biortech.2016.09.130 1 Bioresource Technology, v.222, pp.422 - 430 Bioresource Technology 222 422 430 N scie scopus 000386243600051 2-s2.0-84991094203 Agricultural Engineering Biotechnology & Applied Microbiology Energy & Fuels Agriculture Biotechnology & Applied Microbiology Energy & Fuels Article GENETICALLY-ENGINEERED STRAIN INHIBITORY COMPOUNDS CALCIUM-ION TOLERANCE YEAST ZINC ADAPTATION GLUCOSE GROWTH PH Saccharomyces cerevisiae Xylose fermentation Xylose isomerase Acetic acid Manganese ion