Kim, Myungsuk Kim, Sun Young Randy, Ahmad Lim, Sue Ji Dorjsembe, Banzragch Nho, Chu Won 2024-01-20T03:00:34Z 2024-01-20T03:00:34Z 2021-09-04 2016-12 1756-4646 https://pubs.kist.re.kr/handle/201004/123394 Larix sibirica (Siberian larch) is a deciduous tree native to Russia, Mongolia, and China and has been reported to possess anti-viral, anti-oxidant, and neuroprotective properties. In this study, we assessed the anti-allergic effects of an L. sibirica extract (LSE) and its various flavonoids using an IgE-sensitized mast cell-like cell line, rat basophilic leukaemia (RBL)-2H3, and a passive anaphylaxis mouse model. In IgE-sensitized RBL-2H3 cells, LSE and several flavonoids reduced release and production of beta-hexosaminidase, histamine, and inflammatory mediators (prostaglandin D-2, leukotriene C4, interleuldn-4, and tumour necrosis factor-alpha), reduced expression of cyclooxygenase-2 and 5-lipoxygenase, and suppressed activation of nuclear factor-kappa B. LSE and several flavonoids reduced phosphorylation of downstream signalling molecules, such as Syk, protein kinase C mu, phospholipase C gamma, and mitogen-activated protein kinases. Oral administration of LSE inhibited mast cell-dependent passive cutaneous anaphylactic reactions. These results suggest that LSE is a beneficial candidate for the prevention and treatment of allergic disorders. (C) 2016 Elsevier Ltd. All rights reserved. English ELSEVIER SCIENCE BV PASSIVE CUTANEOUS ANAPHYLAXIS RBL-2H3 CELLS TNF-ALPHA STRUCTURAL REQUIREMENTS SIGNALING PATHWAYS PHENOLIC-COMPOUNDS LEUKOTRIENE C-4 SIBERIAN LARCH KAPPA-B DEGRANULATION Inhibitory effect of the Larix sibirica and its various flavonoids on the IgE-stimulated mast cell activation and anaphylaxis Article 10.1016/j.jff.2016.10.009 1 JOURNAL OF FUNCTIONAL FOODS, v.27, pp.631 - 644 JOURNAL OF FUNCTIONAL FOODS 27 631 644 scie scopus 000390180000054 2-s2.0-84992709468 Food Science & Technology Nutrition & Dietetics Food Science & Technology Nutrition & Dietetics Article PASSIVE CUTANEOUS ANAPHYLAXIS RBL-2H3 CELLS TNF-ALPHA STRUCTURAL REQUIREMENTS SIGNALING PATHWAYS PHENOLIC-COMPOUNDS LEUKOTRIENE C-4 SIBERIAN LARCH KAPPA-B DEGRANULATION Larix sibirica Flavonoids Mast cell RBL-2H3 IgE Fc epsilon RI signalling