Shim, Man Kyu Yoon, Hong Yeol Ryu, Ju Hee Koo, Heebeom Lee, Sangmin Park, Jae Hyung Kim, Jong-Ho Lee, Seulki Pomper, Martin G. Kwon, Ick Chan Kim, Kwangmeyung 2024-01-20T03:01:26Z 2024-01-20T03:01:26Z 2021-09-05 2016-11-14 1433-7851 https://pubs.kist.re.kr/handle/201004/123437 Recently, metabolic glycoengineering with bioorthogonal click reactions has focused on improving the tumor targeting efficiency of nanoparticles as delivery vehicles for anticancer drugs or imaging agents. It is the key technique for developing tumor-specific metabolic precursors that can generate unnatural glycans on the tumor-cell surface. A cathepsin B-specific cleavable substrate (KGRR) conjugated with tri-acetylated N-azidoacetyl-d-mannosamine (RR-SAc3ManNAz) was developed to enable tumor cells to generate unnatural glycans that contain azide groups. The generation of azide groups on the tumor cell surface was exogenously and specifically controlled by the amount of RR-S-Ac(3)ManNAz that was fed to target tumor cells. Moreover, unnatural glycans on the tumor cell surface were conjugated with near infrared fluorescence (NIRF) dye-labeled molecules by a bioorthogonal click reaction in cell cultures and in tumor-bearing mice. Therefore, our RR-S-Ac3ManNAz is promising for research in tumor-specific imaging or drug delivery. English John Wiley & Sons Ltd. FREE CLICK CHEMISTRY BIOORTHOGONAL CHEMISTRY SIALIC-ACID CANCER NANOPARTICLES EXPRESSION PROGRESSION NANOPROBE PATHWAY Cathepsin B-Specific Metabolic Precursor for In Vivo Tumor-Specific Fluorescence Imaging Article 10.1002/anie.201608504 1 Angewandte Chemie International Edition, v.55, no.47, pp.14698 - 14703 Angewandte Chemie International Edition 55 47 14698 14703 scie scopus 000388252700036 2-s2.0-84991744874 Chemistry, Multidisciplinary Chemistry Article FREE CLICK CHEMISTRY BIOORTHOGONAL CHEMISTRY SIALIC-ACID CANCER NANOPARTICLES EXPRESSION PROGRESSION NANOPROBE PATHWAY click chemistry drug delivery imaging agents metabolic glycoengineering tumor targeting