Kim, Hyunju Kang, Dong Hee Koo, Kyung Hee Lee, Seyeong Kim, Seong-Min Kim, Janghwan Yoon, Myung-Han Kim, So Yeon Yang, Eun Gyeong 2024-01-20T03:01:27Z 2024-01-20T03:01:27Z 2021-09-05 2016-11-14 2040-3364 https://pubs.kist.re.kr/handle/201004/123438 The biological applications of vertical nanostructures mostly rely on their intracellular accessibility through the cellular membrane by promoting cell-to-nanostructure interactions. Herein, we report a seemingly counter-intuitive approach for the spontaneous formation of mouse induced pluripotent stem cell (iPSC)-derived three-dimensional spherical colonies with unlimited self-renewal and differentiation potential. The comprehensive analyses of iPSCs cultured on vertical silicon nanocolumn arrays (vSNAs) with various nanocolumn geometries show reduced cell-to-substrate adhesion and enhanced cell-to-cell interactions under optimized vSNA conditions, successfully accommodating the spontaneous production of iPSC-derived spherical colonies. Remarkably, these colonies which were only minimally penetrated by and thereby easily harvested from wafer-sized vSNAs display a substantial increase in pluripotency marker expression and successfully differentiate into three germ layers. Our vSNAs capable of large-scale fabrication, efficient for spherical colony formation, and reusable for multiple iPSC culture could serve as a broad-impact culture platform for stem cell research. English ROYAL SOC CHEMISTRY E-CADHERIN ENDOTHELIAL-CELLS SILICON NANOWIRES DIFFERENTIATION DELIVERY CULTURE INTERROGATION NANONEEDLES MAINTENANCE FIBROBLASTS Vertical nanocolumn-assisted pluripotent stem cell colony formation with minimal cell-penetration Article 10.1039/c6nr06203b 1 NANOSCALE, v.8, no.42, pp.18087 - 18097 NANOSCALE 8 42 18087 18097 scie scopus 000387427400011 2-s2.0-84994165592 Chemistry, Multidisciplinary Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Chemistry Science & Technology - Other Topics Materials Science Physics Article E-CADHERIN ENDOTHELIAL-CELLS SILICON NANOWIRES DIFFERENTIATION DELIVERY CULTURE INTERROGATION NANONEEDLES MAINTENANCE FIBROBLASTS vertical silicon nanocolumn arrays mouse induced pluripotent stem cells spherical colonies stemness