Kang, Joon Hee Lee, Seon-Hyeong Hong, Dongwan Lee, Jae-Seon Ahn, Hee-Sung Ahn, Ju-Hyun Seong, Tae Wha Lee, Chang-Hun Jang, Hyonchol Hong, Kyeong Man Lee, Cheolju Lee, Jae-Ho Kim, Soo-Youl 2024-01-20T03:02:14Z 2024-01-20T03:02:14Z 2021-09-05 2016-11 1226-3613 https://pubs.kist.re.kr/handle/201004/123480 We found that non-small-cell lung cancer (NSCLC) cells express high levels of multiple aldehyde dehydrogenase (ALDH) isoforms via an informatics analysis of metabolic enzymes in NSCLC and immunohistochemical staining of NSCLC clinical tumor samples. Using a multiple reaction-monitoring mass spectrometry analysis, we found that multiple ALDH isozymes were generally abundant in NSCLC cells compared with their levels in normal IMR-90 human lung cells. As a result of the catalytic reaction mediated by ALDH, NADH is produced as a by-product from the conversion of aldehyde to carboxylic acid. We hypothesized that the NADH produced by ALDH may be a reliable energy source for ATP production in NSCLC. This study revealed that NADH production by ALDH contributes significantly to ATP production in NSCLC. Furthermore, gossypol, a pan-ALDH inhibitor, markedly reduced the level of ATP. Gossypol combined with phenformin synergistically reduced the ATP levels, which efficiently induced cell death following cell cycle arrest. English 생화학분자생물학회 HEMATOPOIETIC PROGENITOR CELLS LEUKEMIC HL-60 CELLS LUNG-CANCER GOSSYPOL OXAZAPHOSPHORINES INHIBITION CYCLE EGFR Aldehyde dehydrogenase is used by cancer cells for energy metabolism Article 10.1038/emm.2016.103 1 Experimental & Molecular Medicine, v.48 Experimental & Molecular Medicine 48 scie scopus kci ART002165834 000391820400006 Biochemistry & Molecular Biology Medicine, Research & Experimental Biochemistry & Molecular Biology Research & Experimental Medicine Article HEMATOPOIETIC PROGENITOR CELLS LEUKEMIC HL-60 CELLS LUNG-CANCER GOSSYPOL OXAZAPHOSPHORINES INHIBITION CYCLE EGFR