Han, Hyounkoo Son, Sohee Son, Sejin Kim, Namho Yhee, Ji Young Lee, Jae Hyeop Choi, Jun-Sub Joo, Choun-Ki Lee, Hohyeon Lee, Duhwan Kim, Won Jong Kim, Sun Hwa Kwon, Ick Chan Kim, Hyuncheol Kim, Kwangmeyung 2024-01-20T03:02:24Z 2024-01-20T03:02:24Z 2021-09-05 2016-11 1616-5187 https://pubs.kist.re.kr/handle/201004/123490 The aim of this study is to establish the safe and effective ocular delivery system of therapeutic small interfering RNA (siRNA) in corneal neovascularization therapy. The major hurdle present in siRNA-based corneal neovascularization (CNV) therapy is severe cytotoxicity caused by repetitive drug treatment. A reducible branched polyethylenimine (rBPEI)-based nanoparticle (NP) system is utilized as a new siRNA carrier as a hope for CNV therapy. The thiolated BPEI is readily self-crosslinked in mild conditions to make high molecular weight rBPEI thus allowing the creation of stable siRNA/rBPEI nanoparticles (siRNA-rBPEI-NPs). In the therapeutic region, the rBPEI polymeric matrix is effectively degraded into nontoxic LMW BPEI inside the reductive cytosol causing the rapid release of the encapsulated siRNA into the cytosol to carry out its function. The fluorescent-labeled siRNA-rBPEI-NPs can release siRNA into the entire corneal region after subconjuctival injection into the eye of Sprague Dawley rats thus confirming the proof of concept of this system. English WILEY-V C H VERLAG GMBH NF-KAPPA-B ANGIOGENESIS INHIBITION PEI RNA Reducible Polyethylenimine Nanoparticles for Efficient siRNA Delivery in Corneal Neovascularization Therapy Article 10.1002/mabi.201600051 1 MACROMOLECULAR BIOSCIENCE, v.16, no.11, pp.1583 - 1597 MACROMOLECULAR BIOSCIENCE 16 11 1583 1597 scie scopus 000389084800005 2-s2.0-84982913094 Biochemistry & Molecular Biology Materials Science, Biomaterials Polymer Science Biochemistry & Molecular Biology Materials Science Polymer Science Article NF-KAPPA-B ANGIOGENESIS INHIBITION PEI RNA corneal neovascularization nanoparticles ocular delivery reducible polyethylenimine siRNA