Kim, Suyong Park, Minjoo Yang, Huisuk Dangol, Manita Lahiji, Shayan F. Huh, Inyoung Kim, Miroo Lee, Jaeick Son, Junghyun Jung, Hyungil 2024-01-20T03:02:46Z 2024-01-20T03:02:46Z 2021-09-04 2016-11 0731-7085 https://pubs.kist.re.kr/handle/201004/123510 Dissolving microneedle (DMN), a transdermal drug delivery in which biological drugs are encapsulated in biodegradable and biocompatible polymers, was fabricated using epidermal growth factor (EGF) as a model drug and hyaluronic acid (HA) as a backbone polymeric matrix. After mixing calibration and DMN samples with insulin, an internal standard, solid phase extraction (SPE) was performed to separate EGF and insulin from HA, and then liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS) was conducted for microgram-scale quantitation. The method showed good linearity (R-2 = 0.997) within a specified range (1-4 mu g). Additionally, the decrease in EGF levels during DMN fabrication was compared using the SPE/LC-ESI-MS and enzyme-linked immunosorbent assay (ELISA), a traditional analytical method. The ELISA method detected an EGF loss of only 3.88+4.67%, whereas SPE/LC-ESI-MS detected a loss of 16.75 +/- 4.39%. Qualitative analysis by circular dichroism showed wavelength shift and splitting after DMN fabrication indicating that EGF was denatured during DMN fabrication and cell viability test showed SPE/LC-ESI-MS is more accurate and reliable for detecting the amount of active EGF loaded into the DMN than ELISA. (C) 2016 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV Development of a quantitative method for active epidermal growth factor extracted from dissolving microneedle by solid phase extraction and liquid chromatography electrospray ionization mass spectrometry Article 10.1016/j.jpba.2016.09.002 1 JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v.131, pp.297 - 302 JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 131 297 302 N scie scopus 000387628400039 2-s2.0-84989170902 Chemistry, Analytical Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article STABILITY ACID VACCINE ASSAY Dissolving microneedle Epidermal growth factor Hyaluronic acid Solid phase extraction Liquid chromatography electrospray ionization mass spectrometry Enzyme-linked immunosorbent assay