Kang, D.H. Han, E.H. Jin, C. Kim, H.J. 2024-01-20T03:03:14Z 2024-01-20T03:03:14Z 2021-09-02 2016-11 1226-3311 https://pubs.kist.re.kr/handle/201004/123537 This study aimed to establish an optimal extraction process and high performance liquid chromatography-ultraviolet (HPLC-UV) analytical method for determination of 3,5-dicaffeoylquinic acid (3,5-DCQA) as a part of materials standardization for the development of a xanthine oxidase inhibitor as a health functional food. The quantitative determination method of 3,5-DCQA as a marker compound was optimized by HPLC analysis using a Luna RP-18 column, and the correlation coefficient for the calibration curve showed good linearity of more than 0.9999 using a gradient eluent of water (1% acetic acid) and methanol as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 320 nm. The HPLC-UV method was applied successfully to quantification of the marker compound (3,5-DCQA) in Aster glehni extracts after validation of the method with linearity, accuracy, and precision. Ethanolic extracts of A. glehni (AGEs) were evaluated by reflux extraction at 70 and 80°C with 30, 50, 70, and 80% ethanol for 3, 4, 5, and 6 h, respectively. Among AGEs, 70% AGE at 70°C showed the highest content of 3,5-DCQA of 52.59±3.45 mg/100 g A. glehni. Furthermore, AGEs were analyzed for their inhibitory activities on uric acid production by the xanthine/xanthine oxidase system. The 70% AGE at 70°C showed the most potent inhibitory activity with IC50 values of 77.01±3.13~89.96±3.08 ？g/mL. The results suggest that standardization of 3,5-DCQA in AGEs using HPLC-UV analysis would be an acceptable method for the development of health functional foods. ？ 2016, Korean Society of Food Science and Nutrition. All rights reserved. Korean Korean Society of Food Science and Nutrition Inhibitory effects of ethanolic extracts from Aster glehni on xanthine oxidase and content determination of bioactive components using HPLC-UV Article 10.3746/jkfn.2016.45.11.1610 1 Journal of the Korean Society of Food Science and Nutrition, v.45, no.11, pp.1610 - 1616 Journal of the Korean Society of Food Science and Nutrition 45 11 1610 1616 scopus kci ART002168551 2-s2.0-85007275686 Article 3,5-dicaffeoylquinic acid Aster glehni Health functional foods Quantitative determination Xanthine oxidase inhibitor