Lee, Bo-Ram Ko, Ho Kyung Ryu, Ju Hee Ahn, Keum Young Lee, Young-Ho Oh, Se Jin Na, Jin Hee Kim, Tae Woo Byun, Youngro Kwon, Ick Chan Kim, Kwangmeyung Lee, Jeewon 2024-01-20T03:03:52Z 2024-01-20T03:03:52Z 2021-09-05 2016-10-11 2045-2322 https://pubs.kist.re.kr/handle/201004/123567 Efficient delivery of tumor-specific antigens (TSAs) to lymph nodes (LNs) is essential to eliciting robust immune response for cancer immunotherapy but still remains unsolved. Herein, we evaluated the direct LN-targeting performance of four different protein nanoparticles with different size, shape, and origin [Escherichia coli DNA binding protein (DPS), Thermoplasma acidophilum proteasome (PTS), hepatitis B virus capsid (HBVC), and human ferritin heavy chain (hFTN)] in live mice, using an optical fluorescence imaging system. Based on the imaging results, hFTN that shows rapid LN targeting and prolonged retention in LNs was chosen as a carrier of the model TSA [red fluorescence protein (RFP)], and the flexible surface architecture of hFTN was engineered to densely present RFPs on the hFTN surface through genetic modification of subunit protein of hFTN. The RFP-modified hFTN rapidly targeted LNs, sufficiently exposed RFPs to LN immune cells during prolonged period of retention in LNs, induced strong RFP-specific cytotoxic CD8(+) T cell response, and notably inhibited RFP-expressing melanoma tumor growth in live mice. This suggests that the strategy using protein nanoparticles as both TSA-carrying scaffold and anti-cancer vaccine holds promise for clinically effective immunotherapy of cancer. English NATURE PUBLISHING GROUP PRONE HETEROLOGOUS PROTEINS ESCHERICHIA-COLI PROTEIN DENDRITIC CELL SUBSETS IN-VIVO H-FERRITIN VACCINE-DELIVERY LUNG METASTASIS PEPTIDE ACTIVATION ADJUVANT Engineered Human Ferritin Nanoparticles for Direct Delivery of Tumor Antigens to Lymph Node and Cancer Immunotherapy Article 10.1038/srep35182 1 SCIENTIFIC REPORTS, v.6 SCIENTIFIC REPORTS 6 scie scopus 000384918600001 2-s2.0-84991453496 Multidisciplinary Sciences Science & Technology - Other Topics Article PRONE HETEROLOGOUS PROTEINS ESCHERICHIA-COLI PROTEIN DENDRITIC CELL SUBSETS IN-VIVO H-FERRITIN VACCINE-DELIVERY LUNG METASTASIS PEPTIDE ACTIVATION ADJUVANT