Kang, Bo Ram Yang, Seung-Hoon Chung, Bo-Ryehn Kim, Woong Kim, YoungSoo 2024-01-20T03:03:56Z 2024-01-20T03:03:56Z 2021-09-04 2016-10-07 2045-2322 https://pubs.kist.re.kr/handle/201004/123570 High-grade glioma is a highly malignant and metastatic brain cancer, resistant to many existing anticancer treatments. In such glioma cancer cells, the glucose-regulated protein 78 kDa (GRP78) is particularly highly up-regulated. Former studies have thus targeted mutation-free GRP78 not only to detect glioma cancer cells specifically but also to enhance cytotoxic effect. We focus on cell surface-expressed GRP78 as a target for suppressing high-grade glioma cell lines. Glioblastoma multiforme (GBM) cell line, highly malignant glioma cells, was first injected into 5-week-old athymic mice to confirm and compare GRP78 expression in vivo in xenografted and normal brain tissue. Immunofluorescence and immunoblotting were utilized to detect surface-localized GRP78 in diverse high-grade glioma cell lines. By treating glioma cell lines with the polyclonal N-20 antibody against surface-localized GRP78, we subsequently studied the significance of surface GRP78 to the survival and growth of the glioma cell lines. We found that inhibiting the function of surface GRP78 suppressed cancer cell survival and growth proving that the surface-expressed GRP78 is a vital receptor involved in the proliferation of high-grade glioma. Our findings provide opportunities to target surface GRP78 as a biomarker for high-grade glioma and to develop effective cell-specific anticancer therapy. English NATURE PUBLISHING GROUP ENDOPLASMIC-RETICULUM CHAPERONE RESPONSE REGULATOR GRP78/BIP APOPTOSIS INHIBITORS CANCER INDUCTION PROTEINS BINDING Cell surface GRP78 as a biomarker and target for suppressing glioma cells Article 10.1038/srep34922 1 SCIENTIFIC REPORTS, v.6 SCIENTIFIC REPORTS 6 scie scopus 000384884100001 2-s2.0-84990937731 Multidisciplinary Sciences Science & Technology - Other Topics Article ENDOPLASMIC-RETICULUM CHAPERONE RESPONSE REGULATOR GRP78/BIP APOPTOSIS INHIBITORS CANCER INDUCTION PROTEINS BINDING 뇌종양 brain cancer GRP78