Kim, Do-Hoon Bong, Ji-Hong Yoo, Gu Chang, Seo-Yoon Chang, Young Wook Kang, Min-Jung Jose, Joachim Pyun, Jae-Chul 2024-01-20T03:04:32Z 2024-01-20T03:04:32Z 2021-09-05 2016-10 0141-0229 https://pubs.kist.re.kr/handle/201004/123605 Escherichia coli cells with autodisplayed Z-domains have been used for immunoassays of specific target analytes. In this study, a magnetite suspension was used for the washing step in immunoassays of E. coli cells with autodisplayed Z-domains. This approach enhanced the washing conditions for these immunoassays by determining (1) the optimal concentration of the magnetite suspension, (2) the capacity of the magnetite suspension-based washing method to recover E. coli cells, and (3) the level at which the activity of autodisplayed Z-domains is maintained. In immunoassays of C-reactive protein (CRP), the immunoassay incorporating the magnetite suspension-based washing method showed a sensitivity and limit of detection considerably higher than those of the conventional centrifugation-based washing method. The results indicated that immunoassays incorporating the magnetite suspension-based washing method are effective for medical diagnoses based on CRP assay. (C) 2016 Elsevier Inc. All rights reserved. English ELSEVIER SCIENCE INC PARYLENE-N FILM E. COLI SPR BIOSENSOR BIOMEDICAL APPLICATIONS RECOMBINANT PROTEINS SURFACE DISPLAY OUTER-MEMBRANE IMMOBILIZATION NANOPARTICLES STABILITY A magnetite suspension-based washing method for immunoassays using Escherichia coli cells with autodisplayed Z-domains Article 10.1016/j.enzmictec.2016.06.004 1 ENZYME AND MICROBIAL TECHNOLOGY, v.92, pp.1 - 8 ENZYME AND MICROBIAL TECHNOLOGY 92 1 8 scie scopus 000382798000001 2-s2.0-84975894111 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article PARYLENE-N FILM E. COLI SPR BIOSENSOR BIOMEDICAL APPLICATIONS RECOMBINANT PROTEINS SURFACE DISPLAY OUTER-MEMBRANE IMMOBILIZATION NANOPARTICLES STABILITY Autodisplay Immunoassay Magnetites Washing step