Lee, Joonyeob Han, Gyuseong Shin, Seung Gu Koo, Taewoan Cho, Kyungjin Kim, Woong Hwang, Seokhwan 2024-01-20T03:32:03Z 2024-01-20T03:32:03Z 2021-09-05 2016-09 1385-8947 https://pubs.kist.re.kr/handle/201004/123707 Microbial population size, community structure, and diversity, and the correlations of these characteristics with process variables were investigated in samples taken seasonally over two years from a full-scale thermophilic anaerobic digester treating food waste-recycling wastewater (FRW). The organic component of the FRW consisted of carbohydrate (35% of volatile solids), protein (34%) and lipid (30%). The chemical oxygen demand (COD) removal efficiency of the anaerobic digestion (AD) system negatively correlated with Na+ (2.9-7.7 g/L) and lipid (33-22.8 g/L) concentrations, which varied significantly over the two years. Tepidanaerobacter, Anaerobaculum, Defluviitoga, Keratinibaculum, Gelria, Tepidimicrobium, Caldicoprobacter, Bacillus, and Syntrophaceticus were the major bacterial genera, and Methanoculleus and Methanobacterium were the major archaeal genera. Concentrations of Na+ and lipid in the digester were negatively correlated with total bacterial and archaeal populations determined by real-time quantitative PCR. These concentrations could also significantly affect the bacterial community structure (e.g., negative correlations with Gelria), but not archaeal community structure. Lipid concentration was negatively correlated with bacterial diversity, but was not correlated with archaeal diversity. Ammonia concentration in the digester (2.0-4.3 g N/L) had no significant correlation with COD removal or total bacterial/archaeal populations, but could significantly affect both bacterial and archaeal community structures, including syntrophic acetate-oxidizing bacteria and hydrogenotrophic methanogens. These results indicate that Na+, lipid and ammonia are among the key parameters that affect the process performance of a thermophilic AD system treating FRW and/or the microbial communities in it. (C) 2016 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE SA Seasonal monitoring of bacteria and archaea in a full-scale thermophilic anaerobic digester treating food waste-recycling wastewater: Correlations between microbial community characteristics and process variables Article 10.1016/j.cej.2016.04.097 1 CHEMICAL ENGINEERING JOURNAL, v.300, pp.291 - 299 CHEMICAL ENGINEERING JOURNAL 300 291 299 N scie scopus 000378181400030 2-s2.0-84964886213 Engineering, Environmental Engineering, Chemical Engineering Article SP-NOV. METHANE PRODUCTION GEN. NOV. EMENDED DESCRIPTION DIVERSITY INHIBITION DYNAMICS Anaerobic digestion 454 pyrosequencing Real-time quantitative polymerase chain reaction Sodium inhibition Syntrophic bacteria Hydrogenotrophic methanogens