Lee, Jaehun Choe, Ik Rang Kim, Nak-Kyoon Kim, Won-Je Jang, Hyung-Seok Lee, Yoon-Sik Nam, Ki Tae 2024-01-20T03:32:37Z 2024-01-20T03:32:37Z 2021-09-05 2016-09 1936-0851 https://pubs.kist.re.kr/handle/201004/123740 One of the important challenges in the development of protein-mimetic materials is understanding the sequence-specific assembly behavior and dynamic folding change. Conventional strategies for constructing two-dimensional (2D) nanostructures from peptides have been limited to using beta-sheet forming sequences as building blocks due to their natural tendency to form sheet-like aggregations. We have identified a peptide sequence (YFCFY) that can form dieters via a disulfide bridge, fold into a helix, and assemble into macroscopic flat sheets at the air/water interface. Due to the large driving force for 2D assembly and high elastic modulus of the resulting sheet, the peptide assembly induces flattening of the initially round water droplet. Additionally, we found that stabilization of the helix by dimerization is a key determinant for maintaining macroscopic flatness over a few tens of centimeters even with a uniform thickness of <10 nm. Furthermore, the ability to transfer the sheets from a water droplet to another substrate allows for multiple stacking of 2D peptide nanostructures, suggesting possible applications in biomimetic catalysis, biosensors, and 2D related electronic devices. English AMER CHEMICAL SOC SECONDARY-STRUCTURE PEPTOID NANOSHEETS CIRCULAR-DICHROISM RAMAN-SPECTROSCOPY IN-VIVO NANOMATERIALS ASSEMBLIES PROTEINS CRYSTALS NANOSTRUCTURES Water-Floating Giant Nanosheets from Helical Peptide Pentamers Article 10.1021/acsnano.6b00646 1 ACS NANO, v.10, no.9, pp.8263 - 8270 ACS NANO 10 9 8263 8270 scie scopus 000384399300017 2-s2.0-84989218953 Chemistry, Multidisciplinary Chemistry, Physical Nanoscience & Nanotechnology Materials Science, Multidisciplinary Chemistry Science & Technology - Other Topics Materials Science Article SECONDARY-STRUCTURE PEPTOID NANOSHEETS CIRCULAR-DICHROISM RAMAN-SPECTROSCOPY IN-VIVO NANOMATERIALS ASSEMBLIES PROTEINS CRYSTALS NANOSTRUCTURES peptide self-assembly two-dimensional materials helix disulfide bond biomaterials