Lee, Joonyeob Cho, Kyungjin Shin, Seung Gu Bae, Hyokwan Koo, Taewoan Han, Gyuseong Hwang, Seokhwan 2024-01-20T03:32:44Z 2024-01-20T03:32:44Z 2021-09-04 2016-09 0273-2289 https://pubs.kist.re.kr/handle/201004/123746 This study demonstrated the potential for managing starch processing waste (SPW) by bioconversion to Cordyceps militaris mycelia using solid state cultivation (SSC) and submerged liquid cultivation (SLC). The growth characteristics of C. militaris mycelium were accessed and compared for SSC and SLC systems on SPW under various conditions of initial SPW concentration, pH, and operating temperature. To quantify the mycelial biomass in SLC, original primer sets targeting the 18S rRNA gene of C. militaris were developed. In SSC, a maximum mycelial growth rate (543.1 mm(2)/day) was predicted to occur at 25.6 g SPW/L, pH 5.5, and 23.8 A degrees C. In SLC, a maximum mycelial growth rate (1918.6 mg/L/day) was predicted to occur at 35.5 g SPW/L, pH 5.5, and 22.0 A degrees C. Temperature was suggested as the most significant factor in both systems. The higher optimum substrate concentration observed for SLC than for SSC was likely due to difference in mycelial morphology and mixing effect. English Humana Press, Inc. Nutrient Recovery of Starch Processing Waste to Cordyceps militaris: Solid State Cultivation and Submerged Liquid Cultivation Article 10.1007/s12010-016-2098-4 1 Applied Biochemistry and Biotechnology, v.180, no.2, pp.274 - 288 Applied Biochemistry and Biotechnology 180 2 274 288 N scie scopus 000382675600006 2-s2.0-84964635524 Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Article CULTURE-CONDITIONS METABOLITE PRODUCTION FUNGAL MORPHOLOGY MYCELIAL BIOMASS OPTIMIZATION GROWTH FERMENTATION WATER EXOPOLYSACCHARIDES MUSHROOMS Cordyceps militaris Fungal process Bioconversion Response surface analysis (RSA) Real-time quantitative polymerase chain reaction (QPCR)