Lih, Eugene Park, Ki Wan Chun, So Young Kim, Hyuncheol Kwon, Tae Gyun Joung, Yoon Ki Han, Dong Keun 2024-01-20T03:33:18Z 2024-01-20T03:33:18Z 2021-09-05 2016-08-24 1944-8244 https://pubs.kist.re.kr/handle/201004/123776 Chronic kidney disease is now recognized as a major health problem, but current therapies including dialysis and renal replacement have many limitations. Consequently, biodegradable scaffolds to help repairing injured tissue are emerging as a promising approach in the field of kidney tissue engineering. Poly(lactic-co-glycolic acid) (PLGA) is a useful biomedical material, but its insufficient biocompatibility caused a reduction in cell behavior and function. In this work, we developed the kidney-derived extracellular matrix (ECM) incorporated PLGA scaffolds as a cell supporting material for kidney tissue regeneration. Biomimetic PLGA scaffolds (PLGA/ECM) with different ECM concentrations were prepared by an ice particle leaching method, and their physicochemical and mechanical properties were characterized through various analyses. The proliferation of renal cortical epithelial cells on the PLGA/ECM scaffolds increased with an increase in ECM concentrations (0.2, 1, 5, and 10%) in scaffolds. The PLGA scaffold containing 10% of ECM has been shown to be an effective matrix for the repair and reconstitution of glomerulus and blood vessels in partially nephrectomized mice in vivo, compared with only PLGA control. These results suggest that not only can the tissue-engineering techniques be an effective alternative method for treatment of kidney diseases, but also the ECM incorporated PLGA scaffolds could be promising materials for biomedical applications including tissue engineered scaffolds and biodegradable implants. English American Chemical Society STEM-CELLS GROWTH-FACTORS PORE-SIZE PROLIFERATION STIFFNESS DELIVERY CULTURE DIFFERENTIATION PLATFORM RELEASE Biomimetic Porous PLGA Scaffolds Incorporating Decellularized Extracellular Matrix for Kidney Tissue Regeneration Article 10.1021/acsami.6b03771 1 ACS Applied Materials & Interfaces, v.8, no.33, pp.21145 - 21154 ACS Applied Materials & Interfaces 8 33 21145 21154 scie scopus 000382179400004 2-s2.0-84983528111 Nanoscience & Nanotechnology Materials Science, Multidisciplinary Science & Technology - Other Topics Materials Science Article STEM-CELLS GROWTH-FACTORS PORE-SIZE PROLIFERATION STIFFNESS DELIVERY CULTURE DIFFERENTIATION PLATFORM RELEASE kidney tissue regeneration biomimetic scaffold poly(lactide-co-glycolide) decellularized extracellular matrix ice particle leaching method