Jeong, Eun Sook Kim, Ki Hun Cha, Eunju Kwon, Oh-Seung Cha, Sangwon Lee, Jaeick 2024-01-20T03:34:15Z 2024-01-20T03:34:15Z 2021-09-05 2016-08 1570-0232 https://pubs.kist.re.kr/handle/201004/123829 A rapid and direct paper spray ionization/mass spectrometry (PSI/MS) method was developed for quantitative analysis of ephedrine, pseudoephedrine, norpseudoephedrine, and methylephedrine in human urine. This method involves the use of a triangular filter paper and high-resolution mass spectrometry, where the molecular ions of ephedrines were generated by applying high voltage after loading the spray solvent to the paper which urine sample was pre-loaded. Small amounts (2 mu L) of urine spiked with an internal standard were directly analyzed for ephedrines. The PSI/MS method was validated for linearity, within- and between-run precision, accuracy, and limit of detection. The results showed good linearity (R-2 >= 0.9928) and acceptable precision and accuracy. Furthermore, the accuracy of the method was assessed by analyzing a blind urine sample from World Anti-Doping Agency and comparing the measured concentrations with the nominal concentrations. This test resulted in accuracies ranging from 96.4 to 106.1%, indicating that the PSI/MS method has the potential to be an alternative technique for the fast quantitation of ephedrines in doping control analysis. (C) 2016 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV Direct and rapid quantitation of ephedrines in human urine by paper spray ionization/high resolution mass spectrometry Article 10.1016/j.jchromb.2016.06.040 1 JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.1028, pp.237 - 241 JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 1028 237 241 N scie scopus 000380593600029 2-s2.0-84977671807 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article DOPING CONTROL WHOLE-BLOOD SUBSTANCES DRUGS ABUSE Paper spray ionization/mass spectrometry Ephedrine Doping control analysis