Chwa Jun Won kim wook-jin Sim, Sang Jun Um, Youngsoon Woo, Han Min 2024-01-20T03:34:20Z 2024-01-20T03:34:20Z 2021-09-05 2016-08 1467-7644 https://pubs.kist.re.kr/handle/201004/123834 Capture and conversion of CO2 to valuable chemicals is intended to answer global challenges on environmental issues, climate change and energy security. Engineered cyanobacteria have been enabled to produce industry-relevant chemicals from CO2. However, the final products from cyanobacteria have often been mixed with fermented metabolites during dark fermentation. In this study, our engineering of Synechococcus elongatus PCC 7942 enabled continuous conversion of CO2 to volatile acetone as sole product. This process occurred during lighted, aerobic culture via both ATP-driven malonyl-CoA synthesis pathway and heterologous phosphoketolase (PHK)-phosphotransacetylase (Pta) pathway. Because of strong correlations between the metabolic pathways of acetate and acetone, supplying the acetyl-CoA directly from CO2 in the engineered strain, led to sole production of acetone (22.48mg/L +/- 1.00) without changing nutritional constraints, and without an anaerobic shift. Our engineered S.elongatus strains, designed for acetone production, could be modified to create biosolar cell factories for sustainable photosynthetic production of acetyl-CoA-derived biochemicals. English Blackwell Publishing Inc. Engineering of a modular and synthetic phosphoketolase pathway for photosynthetic production of acetone from CO2 in Synechococcus elongatus PCC 7942 under light and aerobic condition Article 10.1111/pbi.12536 1 Plant Biotechnology Journal, v.14, no.8, pp.1768 - 1776 Plant Biotechnology Journal 14 8 1768 1776 N scie scopus 000379958600011 2-s2.0-84959145501 Biotechnology & Applied Microbiology Plant Sciences Biotechnology & Applied Microbiology Plant Sciences Article ISOPROPANOL PRODUCTION CARBON-DIOXIDE MICROBIAL-PRODUCTION LYSINE ACETYLATION LIMONENE ESCHERICHIA-COLI CYANOBACTERIA CONVERSION METABOLISM Synechococcus elongatus PCC 7942 metabolic engineering acetone biosolar cell factories