Lee, Ga-Yeon Chang, Young Wook Ko, Hyuk Kang, Min-Jung Pyun, Jae-Chul 2024-01-20T04:00:20Z 2024-01-20T04:00:20Z 2021-09-04 2016-07-20 0003-2670 https://pubs.kist.re.kr/handle/201004/123865 A band-type microelectrode was made using a parylene-N film as a passivation layer. A circular-type, mm-scale electrode with the same diameter as the band-type microelectrode was also made with an electrode area that was 5000 times larger than the band-type microelectrode. By comparing the amperometric signals of 3,5,3',5'-tetramethylbenzidine (TMB) samples at different optical density (OD) values, the band-type microelectrode was determined to be 9 times more sensitive than the circular-type electrode. The properties of the circular-type and the band-type electrodes (e.g., the shape of their cyclic voltammograms, the type of diffusion layer used, and the diffusion layer thickness per unit electrode area) were characterized according to their electrode area using the COMSOL Multiphysics software. From these simulations, the band-type electrode was estimated to have the conventional microelectrode properties, even when the electrode area was 100 times larger than a conventional circular-type electrode. These results show that both the geometry and the area of an electrode can influence the properties of the electrode. Finally, amperometric analysis based on a band-type electrode was applied to commercial ELISA kits to analyze human hepatitis B surface antigen (hHBsAg) and human immunodeficiency virus (HIV) antibodies. (C) 2016 Elsevier B.V. All rights reserved. English ELSEVIER SPR BIOSENSOR PARYLENE ELECTRODE SENSOR LAYER FILM Band-type microelectrodes for amperometric immunoassays Article 10.1016/j.aca.2016.05.009 1 ANALYTICA CHIMICA ACTA, v.928, pp.39 - 48 ANALYTICA CHIMICA ACTA 928 39 48 scie scopus 000376656700004 2-s2.0-84969522973 Chemistry, Analytical Chemistry Article SPR BIOSENSOR PARYLENE ELECTRODE SENSOR LAYER FILM Microelectrode Band-type Parylene Amperometry Simulation Immunoassay