Park, Subin Lee, Jangwook Jo, Mi-hee Na, Jin Hee Park, Sung-Gurl Jang, Hyeon-Ki Kang, Sun-Woong Kim, Jong-Ho Kim, Byung-Soo Park, Jae Hyung Kwon, Ick Chan Ryu, Ju Hee Kim, Kwangmeyung 2024-01-20T04:00:59Z 2024-01-20T04:00:59Z 2021-09-05 2016-07 0939-4451 https://pubs.kist.re.kr/handle/201004/123897 Vascular endothelial growth factor receptor (VEGFR) and matrix metalloproteinase (MMP) are up-regulated in ischemic tissue and play pivotal roles in promoting angiogenesis. The purpose of the present study was to evaluate two fluorophore-conjugated peptide probes specific to VEGFR and MMP for dual-targeted in vivo monitoring of angiogenesis in a murine model of hindlimb ischemia. To this end, VEGFR-Probe and MMP-Probe were developed by conjugating distinct near-infrared fluorophores to VEGFR-binding and MMP substrate peptides, respectively. VEGFR-Probe exhibited specific binding to VEGFR on HUVECs, and self-quenched MMP-Probe produced strong fluorescence intensity in the presence of MMPs in vitro. Subsequently, VEGFR-Probe and MMP-Probe were successfully utilized for time course in vivo visualization of VEGFR or MMP, respectively. Simultaneous visualization provided information regarding the spatial distribution of these proteins, including areas of co-localization. This dual-targeted in vivo imaging approach will be useful for understanding the detailed mechanism of angiogenesis and for evaluating therapeutic angiogenesis. English SPRINGER WIEN GLYCOL CHITOSAN NANOPARTICLES ENDOTHELIAL GROWTH-FACTOR MATRIX-METALLOPROTEINASE ACTIVITY VEGF RECEPTORS CANCER-THERAPY DIAGNOSIS TISSUE VASCULATURE DEGRADATION DYSFUNCTION In vivo monitoring of angiogenesis in a mouse hindlimb ischemia model using fluorescent peptide-based probes Article 10.1007/s00726-016-2225-0 1 AMINO ACIDS, v.48, no.7, pp.1641 - 1654 AMINO ACIDS 48 7 1641 1654 scie scopus 000377409900010 2-s2.0-84964374593 Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article GLYCOL CHITOSAN NANOPARTICLES ENDOTHELIAL GROWTH-FACTOR MATRIX-METALLOPROTEINASE ACTIVITY VEGF RECEPTORS CANCER-THERAPY DIAGNOSIS TISSUE VASCULATURE DEGRADATION DYSFUNCTION Vascular endothelial growth factor receptor Matrix metalloproteinase Molecular imaging Angiogenesis Fluorescence imaging