Lee, Su Hyeon Kim, Eung Ju Lee, Dong-Hyoung Lee, Won-Yong Chung, Bong Chul Seo, Hong Seog Choi, Man Ho 2024-01-20T04:01:11Z 2024-01-20T04:01:11Z 2021-09-04 2016-07 2234-3806 https://pubs.kist.re.kr/handle/201004/123908 Background: Eicosanoids are metabolites of arachidonic acid that are rapidly biosynthesized and degraded during inflammation, and their metabolic changes reveal altered enzyme expression following drug treatment. We developed an eicosanoid profiling method and evaluated their changes on drug treatment. Methods: Simultaneous quantitative profiling of 32 eicosanoids in liver S9 fractions obtained from rabbits with carrageenan-induced inflammation was performed and validated by liquid chromatography-mass spectrometry coupled to anion-exchange solid-phase purification. Results: The limit of quantification for the devised method ranged from 0.5 to 20.0 ng/mg protein, and calibration linearity was achieved (R-2>0.99). The precision (% CV) and accuracy (% bias) ranged from 4.7 to 10.3% and 88.4 to 110.9%, respectively, and overall recoveries ranged from 58.0 to 105.3%. Our method was then applied and showed that epitestosterone treatment reduced the levels of all eicosanoids that were generated by cyclooxygenases and lipoxygenases. Conclusions: Quantitative eicosanoid profiling combined with in vitro metabolic assays may be useful for evaluating metabolic changes affected by drugs during eicosanoid metabolism. English KOREAN SOC LABORATORY MEDICINE LC-MS/MS INFLAMMATION TESTOSTERONE DEHYDROEPIANDROSTERONE EPITESTOSTERONE LIPIDOMICS OXYLIPINS MEDIATORS CYTOKINES Liquid Chromatography-Mass Spectrometry-Based In Vitro Metabolic Profiling Reveals Altered Enzyme Expressions in Eicosanoid Metabolism Article 10.3343/alm.2016.36.4.342 1 ANNALS OF LABORATORY MEDICINE, v.36, no.4, pp.342 - 352 ANNALS OF LABORATORY MEDICINE 36 4 342 352 scie scopus kci ART002120912 000376049300008 2-s2.0-84969221032 Medical Laboratory Technology Medical Laboratory Technology Article LC-MS/MS INFLAMMATION TESTOSTERONE DEHYDROEPIANDROSTERONE EPITESTOSTERONE LIPIDOMICS OXYLIPINS MEDIATORS CYTOKINES Eicosanoids Arachidonic acid Liquid chromatography-mass spectrometry Liver S9 fraction Epitestosterone