Chae, Dong-Kyu Ban, Eunmi Yoo, Young Sook Baik, Ja-Hyun Song, Eun Joo 2024-01-20T04:01:19Z 2024-01-20T04:01:19Z 2021-09-04 2016-07 1618-2642 https://pubs.kist.re.kr/handle/201004/123916 MicroRNAs (miRNAs) are short RNA molecules that control the expression of mRNAs associated with various biological processes. Therefore, deregulated miRNAs play an important role in the pathogenesis of diseases. Numerous studies aimed at developing novel miRNA-based drugs or determining miRNA functions have been conducted by inhibiting miRNAs using anti-miRNA oligonucleotides (AMOs), which inhibit the function by hybridizing with miRNA. To increase the binding affinity and specificity to target miRNA, AMOs with various chemical modifications have been developed. Evaluating the potency of these various types of AMOs is an essential step in their development. In this study, we developed a capillary electrophoresis with laser-induced fluorescence (CE-LIF) method to evaluate the potency of AMOs by measuring changes in miRNA levels with fluorescence-labeled ssDNA probes using AMO-miR-23a, which inhibits miR-23a related to lung cancer. In order to eliminate interference by excess AMOs during hybridization of the ssDNA probe with the miR-23a, the concentration of the ssDNA probe was optimized. This newly developed method was used to compare the potency of two different modified AMOs. The data were supported by the results of a luciferase assay. This study demonstrated that CE-LIF analysis could be used to accurately evaluate AMO potency in biological samples. English SPRINGER HEIDELBERG CAPILLARY-ELECTROPHORESIS QUANTITATIVE-ANALYSIS MICRORNAS CANCER DESIGN SERUM Evaluation of inhibition of miRNA expression induced by anti-miRNA oligonucleotides Article 10.1007/s00216-016-9611-z 1 ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.408, no.18, pp.4829 - 4833 ANALYTICAL AND BIOANALYTICAL CHEMISTRY 408 18 4829 4833 scie scopus 000378999500002 2-s2.0-84968639128 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article CAPILLARY-ELECTROPHORESIS QUANTITATIVE-ANALYSIS MICRORNAS CANCER DESIGN SERUM miRNA Anti-miRNA oligonucleotide Capillary electrophoresis with laser-induced fluorescence miR-23a