Park, Min Hee Song, Boa Hong, Seungpyo Kim, Sang Heon Lee, Kangwon 2024-01-20T04:01:27Z 2024-01-20T04:01:27Z 2021-09-04 2016-07 1543-8384 https://pubs.kist.re.kr/handle/201004/123924 Invasion and metastasis of cancer directly related to human death have been associated with interactions among many different types of cells and three-dimensional (3D) tissue matrices. Precise mechanisms related to cancer invasion and metastasis still remain unknown due to their complexities. Development of tumor microenvironment (TME)-mimicking system could play a key role in understanding cancer environments and in elucidating the relating phenomena and their driving forces. Here we report a facile and novel platform of 3D cancer cell-clusters using human adipose-derived mesenchymal stem cells (hASCs) and breast cancer cells (MDA-MB-231) within a collagen gel matrix to show cancer invasion in the cell and extracellular matrix (ECM). Both clusters A (hASC only) and AC (hASC and MDA-MB-231) exhibited different behaviors and expressions of migration and invasion, as observed by the relating markers such as fibronectin, alpha-SMA, and CXCR4. hASCs showed a protrusive migration from a cluster center, whereas MDA-MB-231 spread out radially followed by hASC migration. Finally, the effect of matrix was further discussed by varying collagen gel densities. The new biomimetic system of 3D cancer clusters developed here has the potential to be utilized for research on migration and invasion of cancer cells in extracellular matrices. English AMER CHEMICAL SOC FIBROBLAST-GROWTH-FACTOR TUMOR HETEROGENEITY MATRIX STIFFNESS TISSUE METASTASIS ADHESION MORPHOGENESIS BINDING DIFFERENTIATION REGENERATION Biomimetic 3D Clusters Using Human Adipose Derived Mesenchymal Stem Cells and Breast Cancer Cells: A Study on Migration and Invasion of Breast Cancer Cells Article 10.1021/acs.molpharmaceut.5b00953 1 MOLECULAR PHARMACEUTICS, v.13, no.7, pp.2204 - 2213 MOLECULAR PHARMACEUTICS 13 7 2204 2213 scie scopus 000379455800009 2-s2.0-84979017520 Medicine, Research & Experimental Pharmacology & Pharmacy Research & Experimental Medicine Pharmacology & Pharmacy Article FIBROBLAST-GROWTH-FACTOR TUMOR HETEROGENEITY MATRIX STIFFNESS TISSUE METASTASIS ADHESION MORPHOGENESIS BINDING DIFFERENTIATION REGENERATION cell cluster collagen gel migration invasion tumor microenvironment