Lee, Hyungbeen Lee, Sang Won Lee, Gyudo Lee, Wonseok Lee, Jeong Hoon Hwang, Kyo Seon Yang, Jaemoon Lee, Sang Woo Yoon, Dae Sung 2024-01-20T04:01:35Z 2024-01-20T04:01:35Z 2021-09-03 2016-07 2040-3364 https://pubs.kist.re.kr/handle/201004/123930 Kelvin probe force microscopy (KPFM) is a robust toolkit for profiling the surface potential (SP) of biomolecular interactions between DNAs and/or proteins at the single molecule level. However, it has often suffered from background noise and low throughput due to instrumental or environmental constraints, which is regarded as limiting KPFM applications for detection of minute changes in the molecular structures such as single-nucleotide polymorphism (SNP). Here, we show KPFM imaging of DNA-capped nanoparticles (DCNP) that enables SNP detection of the BRCA1 gene owing to sterically well-adjusted DNA-DNA interactions that take place within the confined spaces of DCNP. The average SP values of DCNP interacting with BRCA1 SNP were found to be lower than the DCNP reacting with normal (non-mutant) BRCA1 gene. We also demonstrate that SP characteristics of DCNP with different substrates (e.g., Au, Si, SiO2, and Fe) provide us with a chance to attenuate or augment the SP signal of DCNP without additional enhancement of instrumentation capabilities. English ROYAL SOC CHEMISTRY GOLD NANOPARTICLES CARBON NANOTUBE WORK FUNCTION LABEL-FREE FILMS FIBRILS CANCER SENSOR LAYER IONS Kelvin probe force microscopy of DNA-capped nanoparticles for single-nucleotide polymorphism detection Article 10.1039/c5nr08969g 1 NANOSCALE, v.8, no.28, pp.13537 - 13544 NANOSCALE 8 28 13537 13544 scie scopus 000380021100001 2-s2.0-84978722203 Chemistry, Multidisciplinary Nanoscience & Nanotechnology Materials Science, Multidisciplinary Physics, Applied Chemistry Science & Technology - Other Topics Materials Science Physics Article GOLD NANOPARTICLES CARBON NANOTUBE WORK FUNCTION LABEL-FREE FILMS FIBRILS CANCER SENSOR LAYER IONS