Moon, Pyong-Gon Lee, Jeong-Eun Cho, Young-Eun Lee, Soo Jung Chae, Yee Soo Jung, Jin Hyang Kim, In-San Park, Ho Yong Baek, Moon-Chang 2024-01-20T04:01:54Z 2024-01-20T04:01:54Z 2021-09-05 2016-06-28 1949-2553 https://pubs.kist.re.kr/handle/201004/123944 Extracellular vesicles (EVs) secreted from cancer cells have potential for generating cancer biomarker signatures. Fibronectin (FN) was selected as a biomarker candidate, due to the presence in surface on EVs secreted from human breast cancer cell lines. A subsequent study used two types of enzyme-linked immunosorbent assays (ELISA) to determine the presence of these proteins in plasma samples from disease-free individuals (n=70), patients with BC (n=240), BC patients after surgical resection (n=40), patients with benign breast tumor (n=55), and patients with non-cancerous diseases (thyroiditis, gastritis, hepatitis B, and rheumatoid arthritis; n=80). FN levels were significantly elevated (p<.0001) at all stages of BC, and returned to normal after tumor removal. The diagnostic accuracy for FN detection in extracellular vesicles (ELISA method 1) (area under the curve, 0.81; 95% CI, 0.76 to 0.86; sensitivity of 65.1% and specificity of 83.2%) were also better than those for FN detection in the plasma (ELISA method 2) (area under the curve, 0.77; 95% CI, 0.72 to 0.83; sensitivity of 69.2% and specificity of 73.3%) in BC. The diagnostic accuracy of plasma FN was similar in both the early-stage BC and all BC patients, as well as in the two sets. This liquid biopsy to detect FN on circulating EVs could be a promising method to detect early breast cancer. English IMPACT JOURNALS LLC CELL-MIGRATION INTEGRIN REGULATION URINARY EXOSOMES OVARIAN-CANCER MICROVESICLES INVASION METASTASIS BIOMARKERS PROTEOMICS PATHWAYS Fibronectin on circulating extracellular vesicles as a liquid biopsy to detect breast cancer Article 10.18632/oncotarget.9561 1 ONCOTARGET, v.7, no.26, pp.40189 - 40199 ONCOTARGET 7 26 40189 40199 scie scopus 000378614700096 2-s2.0-84982995063 Oncology Cell Biology Oncology Cell Biology Article CELL-MIGRATION INTEGRIN REGULATION URINARY EXOSOMES OVARIAN-CANCER MICROVESICLES INVASION METASTASIS BIOMARKERS PROTEOMICS PATHWAYS breast cancer diagnosis extracellular vesicle ELISA