Lee, Young-Sun Bae, Yeonju Park, Nammi Yoo, Jae Cheal Cho, Chang-Hoon Ryoo, Kanghyun Hwang, Eun Mi Park, Jae-Yong 2024-01-20T04:02:02Z 2024-01-20T04:02:02Z 2021-09-05 2016-06-24 0006-291X https://pubs.kist.re.kr/handle/201004/123952 Anoctamin-1 (ANO1) is a Ca2+-activated chloride channel (CaCC) that plays important physiological roles in normal and cancerous tissues. However, the plasma membrane trafficking mechanisms of ANO1 remain poorly characterized. In yeast two-hybrid screening experiments, we observed direct interactions of ANO1 with beta-COP, which is a subunit of Coat Protein Complex I (COPI). This interaction was then confirmed using several in vitro and in vivo binding assays. Moreover, the cotransfection of beta-COP with ANO1 into HEK293T cells led to decreased the surface expression and the channel activity of ANO1. Accordingly, endogenous ANO1 was associated with beta-COP in U251 glioblastoma cells, and silencing of beta-COP enhanced surface expression and whole-cell currents of ANO1 in these cells. Taken together, these data suggest that beta-COP negatively regulates ANO1 surface expression. (C) 2016 Elsevier Inc. All rights reserved. English ACADEMIC PRESS INC ELSEVIER SCIENCE CANCER PROGRESSION TMEM16A CELLS CONDUCTANCE CARCINOMA MARKER Surface expression of the Anoctamin-1 (ANO1) channel is suppressed by protein-protein interactions with beta-COP Article 10.1016/j.bbrc.2016.05.077 1 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.475, no.2, pp.216 - 222 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 475 2 216 222 scie scopus 000378020700011 2-s2.0-84969584401 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article CANCER PROGRESSION TMEM16A CELLS CONDUCTANCE CARCINOMA MARKER ANO1 beta-COP Surface expression Yeast two-hybrid screening Protein-protein interactions U251 glioblastoma cells