Kuzmin, Andrey N. Pliss, Artem Lim, Chang-Keun Heo, Jeongyun Kim, Sehoon Rzhevskii, Alexander Gu, Bobo Yong, Ken-Tye Wen, Shangchun Prasad, Paras N. 2024-01-20T04:02:03Z 2024-01-20T04:02:03Z 2021-09-05 2016-06-24 2045-2322 https://pubs.kist.re.kr/handle/201004/123953 Raman microspectroscopy provides for high-resolution non-invasive molecular analysis of biological samples and has a breakthrough potential for dissection of cellular molecular composition at a single organelle level. However, the potential of Raman microspectroscopy can be fully realized only when novel types of molecular probes distinguishable in the Raman spectroscopy modality are developed for labeling of specific cellular domains to guide spectrochemical spatial imaging. Here we report on the design of a next generation Raman probe, based on BlackBerry Quencher 650 compound, which provides unprecedentedly high signal intensity through the Resonance Raman (RR) enhancement mechanism. Remarkably, RR enhancement occurs with low-toxic red light, which is close to maximum transparency in the biological optical window. The utility of proposed RR probes was validated for targeting lysosomes in live cultured cells, which enabled identification and subsequent monitoring of dynamic changes in this organelle by Raman imaging. English NATURE PUBLISHING GROUP SPECTROSCOPY MICROSCOPY SERS DNA TRANSFORMATIONS NUCLEOLI PROTEOME Resonance Raman Probes for Organelle-Specific Labeling in Live Cells Article 10.1038/srep28483 1 SCIENTIFIC REPORTS, v.6 SCIENTIFIC REPORTS 6 scie scopus 000378599100001 2-s2.0-84976416698 Multidisciplinary Sciences Science & Technology - Other Topics Article SPECTROSCOPY MICROSCOPY SERS DNA TRANSFORMATIONS NUCLEOLI PROTEOME