Jeong, Ga Ram Im, Sun-Kyoung Bae, Yun-Hee Park, Eun Su Jin, Byung Kwan Kwon, Hyug Moo Lee, Beom-Joon Bu, Youngmin Hur, Eun-Mi Lee, Byoung Dae 2024-01-20T04:02:15Z 2024-01-20T04:02:15Z 2021-09-05 2016-06-15 0165-5728 https://pubs.kist.re.kr/handle/201004/123965 Tonicity-responsive enhancer (TonE) binding protein (TonEBP) is known as an osmosensitive transcription factor that regulates cellular homeostasis during states of hypo- and hypertonic stress. In addition to its role in osmoadaptation, growing lines of evidence suggest that TonEBP might have tonicity-independent functions. In particular, a number of studies suggest that inflammatory stimuli induce the expression and activation of TonEBP in peripheral immune cells. However, whether TonEBP is expressed in microglia, resident immune cells of the central nervous system, is unknown. Here we show that inflammatory signals induce the expression of TonEBP in microglia both in vitro and in vitro. In cultured primary microglia, treatment with lipopolysaccharide (LPS), interferon-gamma, and interleukin 4 increased the expression of TonEBP. Moreover, we found that stereotaxic injection of LPS into the substantia nigra region of rat brain increased TonEBP expression in OX-42-positive cells. Further-more, expression of TonEBP was induced in OX-42-positive cells in a rat model of transient middle cerebral artery occlusion. Together these results show that the expression of TonEBP is regulated by inflammatory signals in mammalian brain, suggesting that TonEBP might play a part during neuroinflammation. (C) 2016 The Authors. Published by Elsevier B.V. English ELSEVIER SCIENCE BV LIPOPOLYSACCHARIDE-INDUCED NEURODEGENERATION TRANSCRIPTION FACTOR TONEBP SYSTEMIC HYPERTONICITY RAT-BRAIN IN-VIVO NFAT5 GENE NEUROINFLAMMATION INHIBITION Inflammatory signals induce the expression of tonicity-responsive enhancer binding protein (TonEBP) in microglia Article 10.1016/j.jneuroim.2016.04.009 1 JOURNAL OF NEUROIMMUNOLOGY, v.295, pp.21 - 29 JOURNAL OF NEUROIMMUNOLOGY 295 21 29 scie scopus 000377822000005 2-s2.0-84963956577 Immunology Neurosciences Immunology Neurosciences & Neurology Article LIPOPOLYSACCHARIDE-INDUCED NEURODEGENERATION TRANSCRIPTION FACTOR TONEBP SYSTEMIC HYPERTONICITY RAT-BRAIN IN-VIVO NFAT5 GENE NEUROINFLAMMATION INHIBITION Microglia TonEBP LPS Neuroinflammation