Park, Jong-Min Jung, Ha-Wook Chang, Young Wook Kim, Hyung-Seok Kang, Min-Jung Pyun, Jae-Chul 2024-01-20T04:02:30Z 2024-01-20T04:02:30Z 2022-01-25 2016-06 1475-7435 https://pubs.kist.re.kr/handle/201004/123979 In this work, Pt nanoparticles were synthesised according to the citrate reduction process. The physical properties of these Pt nanoparticles were characterised using dynamic laser scattering (DLS), zeta-potential analysis, thermal gravimetric analysis (TGA), and TEM. The peroxidase activity of the Pt nanoparticles was also analysed using a chromogenic substrate (TMB) and a luminescent probe (luminol). For application to immunoassays, Pt nanoparticles were coupled with antibodies via electrostatic interaction, and the coupling conditions were optimised at different pH conditions. Finally, the applicability of Pt nanoparticles coupled with anti-hCG (human chorionic gonadotropin) antibodies was demonstrated for the immunoassay of hCG for the medical diagnosis of pregnancy. English INDERSCIENCE ENTERPRISES LTD Immunoassays based on Pt nanoparticles with peroxidase activity Article 10.1504/IJNT.2016.077090 1 INTERNATIONAL JOURNAL OF NANOTECHNOLOGY, v.13, no.4-6, pp.402 - 412 INTERNATIONAL JOURNAL OF NANOTECHNOLOGY 13 4-6 402 412 N scie scopus 000378609500016 2-s2.0-84975503697 Nanoscience & Nanotechnology Materials Science, Multidisciplinary Science & Technology - Other Topics Materials Science Article COLI Pt nanoparticle immunoassay antibody coupling hCG