Gil, Chang-Hyun Ki, Byeong-Seong Seo, Joseph Choi, Jong-Jin Kim, Hana Kim, In-Gul Jung, A-Ra Lee, Won-Young Choi, Youngsok Park, Kwideok Moon, Sung-Hwan Chung, Hyung-Min 2024-01-20T04:02:32Z 2024-01-20T04:02:32Z 2022-01-25 2016-06 1738-2696 https://pubs.kist.re.kr/handle/201004/123981 Hemangioblasts or blood islands only arise in early development thereby the sources to obtain these bi-potential cells are limited. While previous studies have isolated both lineages in vitro through the hemangioblast, derivation efficiency was rather low due to cellular damage attributed by enzyme usage and fluorescent activated cell sorting (FACS). This study focused on avoiding the use of damaging factors in the derivation of endothelial cells (ECs). Single cell H9-human embryonic stem cells (hESCs) were obtained by using a mild dissociation protocol then human embryoid body (hEB) formation was performed under hemangioblast differentiation conditions. The hEBs were subjected to a two-stage cytokine treatment procedure. Subsequent culture of the adhesive cells in day 4 hEBs gave arise to a seemingly pure population of ECs. The hESC-derived ECs were characterized by identifying signature endothelial gene and protein markers as well as testing for in vitro functionality. Furthermore, in vivo functionality was also confirmed by transplanting the cells in hindlimb ischemic murine models. We demonstrate that the genetic change required for EC derivation precedes blast colony formation. Furthermore, cell damage was prevented by abating enzyme usage and FACS, resulting in a high yield of ECs upon adhesion. Under this method, confluent cultures of ECs were obtainable 4 days after hEB formation which is significantly faster than previous protocols. English KOREAN TISSUE ENGINEERING REGENERATIVE MEDICINE SOC Directing Human Embryonic Stem Cells towards Functional Endothelial Cells Easily and without Purification Article 10.1007/s13770-016-9076-3 1 TISSUE ENGINEERING AND REGENERATIVE MEDICINE, v.13, no.3, pp.274 - 283 TISSUE ENGINEERING AND REGENERATIVE MEDICINE 13 3 274 283 N scie scopus kci ART002112158 000377468200009 2-s2.0-84973333538 Cell & Tissue Engineering Engineering, Biomedical Cell Biology Engineering Article COMMON PRECURSOR HEMANGIOBLAST HEMATOPOIESIS NEOVASCULARIZATION TRANSPLANTATION EMBRYOGENESIS PROGENITOR Differentiation Endothelial cell Embryonic stem cell Embryoid body Hemangioblast